341595 | FGF-2, Basic, Human, Recombinant, E. coli

341595
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥97% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      341595-25UG
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          Plastic ampoule 25 μg
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          Description
          OverviewRecombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells.
          Catalogue Number341595
          Brand Family Calbiochem®
          SynonymsrhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli
          References
          ReferencesGoureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
          Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
          Li, J.J., et al. 1993. Cancer 72, 2253.
          Mignatti, P., et al. 1992. J. Cell Physiol. 151, 81.
          Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 11007.
          Baird, A., and Klagsbrun, M. 1991. Cancer Cells 3, 239.
          Gospodarowicz, D. 1991. Ann. NY Acad. Sci. 638, 1.
          Basilico, C., et al. 1989. Ann. NY Acad. Sci. 567, 95.
          Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem. 58, 575.
          Rogelj, S., et al. 1989. J. Cell. Biochem. 39, 13.
          Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
          Abraham, J.A., et al. 1986. EMBO J. 5, 2523.
          Product Information
          CAS number106096-93-9
          FormLyophilized
          FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0.
          Applications
          Biological Information
          Biological activityED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
          Purity≥97% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1 EU/µg FGF
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSLK3641325
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 6 months at -70°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          341595

          References

          Reference overview
          Goureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
          Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
          Li, J.J., et al. 1993. Cancer 72, 2253.
          Mignatti, P., et al. 1992. J. Cell Physiol. 151, 81.
          Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 11007.
          Baird, A., and Klagsbrun, M. 1991. Cancer Cells 3, 239.
          Gospodarowicz, D. 1991. Ann. NY Acad. Sci. 638, 1.
          Basilico, C., et al. 1989. Ann. NY Acad. Sci. 567, 95.
          Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem. 58, 575.
          Rogelj, S., et al. 1989. J. Cell. Biochem. 39, 13.
          Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
          Abraham, J.A., et al. 1986. EMBO J. 5, 2523.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Nitric Oxide and Oxidative Stress Brochure
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-May-2008 RFH
          SynonymsrhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli
          DescriptionRecombinant, human basic fibroblast growth factor expressed in E. coli. A member of the FGF family of mitogenic peptides, comprised of seven related proteins showing 35-55% amino acid conservation within the family and 55% similarity with acidic FGF. Found in neuronal tissue (hippocampus, brain stem, peripheral ganglia, and the isocortex), pituitary, adrenal cortex, corpus luteum, and placenta. Some of the biological activities attributed to basic FGF include: mitogenic activity in vitro for cells of meso- and neuroectodermal origin (i.e. fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, bovine epithelial lens cells, osteoblasts, smooth muscle cells, and melanocytes); role in wound healing; induction of neuron differentiation, survival, and regeneration; chemoattractant effect and mitogenic activity in vitro for endothelial cells and angiogenic activity in vivo; role in tumor neovascularization. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits iNOS in bovine retinal pigmented epithelial cells. Binding of FGF to heparin or cell surface heparin sulfate proteoglycans is necessary for FGF binding to its high affinity receptors. Basic and acidic FGF appear to bind to the same high affinity receptors and show a similar range of biological activities.
          FormLyophilized
          FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0.
          CAS number106096-93-9
          RTECSLK3641325
          Purity≥97% by SDS-PAGE
          ContaminantsEndotoxin: ≤1 EU/µg FGF
          Biological activityED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
          SolubilityReconstitute to a concentration ≥10 µg/ml with sterile PBS containing 1 mM DTT and ≥0.1% HSA or BSA.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 6 months at -70°C.
          Toxicity Standard Handling
          Merck USA index14, 4072
          ReferencesGoureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
          Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
          Li, J.J., et al. 1993. Cancer 72, 2253.
          Mignatti, P., et al. 1992. J. Cell Physiol. 151, 81.
          Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 11007.
          Baird, A., and Klagsbrun, M. 1991. Cancer Cells 3, 239.
          Gospodarowicz, D. 1991. Ann. NY Acad. Sci. 638, 1.
          Basilico, C., et al. 1989. Ann. NY Acad. Sci. 567, 95.
          Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem. 58, 575.
          Rogelj, S., et al. 1989. J. Cell. Biochem. 39, 13.
          Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
          Abraham, J.A., et al. 1986. EMBO J. 5, 2523.

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          Categories

          Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
          Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors