324762 | Endopeptidase, Neutral, Porcine Kidney

324762
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324762-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewNative, neutral endopeptidase from porcine kidney. A cell surface, zinc containing metallopeptidase that is abundant in the brush border membrane of the kidney proximal tubules. Also present in brain, endothelial cells, liver, and lung. Peptidase activity is specifically directed toward the cleavage on the amino side of hydrophobic residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.
          Catalogue Number324762
          Brand Family Calbiochem®
          SynonymsCD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin
          References
          ReferencesBarnes, K., et al. 1995. J. Neurochem. 64, 1826.
          Gafford, J.T., et al. 1983. Biochemistry 22, 3265.
          Product Information
          Unit of DefinitionOne unit of enzyme will liberate 1.0 µmol <i>p</i>NA per min from Glutaryl-(Ala)₂-Phe-<i>p</i>NA at 25°C, pH 7.4.
          EC number3.4.24.11
          FormLyophilized
          FormulationLyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON® X-100 Detergent, pH 7.4.
          Applications
          Biological Information
          Purity≥95% by HPLC
          Specific Activity≥850 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324762

          References

          Reference overview
          Barnes, K., et al. 1995. J. Neurochem. 64, 1826.
          Gafford, J.T., et al. 1983. Biochemistry 22, 3265.

          Brochure

          Title
          Proteases Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-June-2008 RFH
          SynonymsCD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin
          DescriptionNative, neutral endopeptidase from porcine kidney. A cell surface zinc-containing proteinase that is abundant in the brush border membranes of the kidney proximal tubules. Also present in the brain, endothelial cells, liver, and the lung tissue. Peptidase activity is specifically directed towards the cleavage on the amino side of hydrophobic amino acid residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.
          FormLyophilized
          FormulationLyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON® X-100 Detergent, pH 7.4.
          Recommended reaction conditionsSee Gafford, J. T., et al. 1983 (below)
          EC number3.4.24.11
          Purity≥95% by HPLC
          Specific activity≥850 units/mg protein
          Unit definitionOne unit of enzyme will liberate 1.0 µmol pNA per min from Glutaryl-(Ala)₂-Phe-pNA at 25°C, pH 7.4.
          SolubilityPhysiological buffers, pH 7.4 (e.g., Tris-buffered saline) (1 mg/ml)
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.
          Toxicity Standard Handling
          ReferencesBarnes, K., et al. 1995. J. Neurochem. 64, 1826.
          Gafford, J.T., et al. 1983. Biochemistry 22, 3265.