324856 | EGF, Murine Submaxillary Glands, Receptor Grade

324856
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥98% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324856-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewNative epidermal growth factor from mouse submaxillary glands. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Purified by size-exclusion chromatography followed by DEAE ion-exchange chromatography.
          Effective biological range: 5-50 ng/ml.
          Catalogue Number324856
          Brand Family Calbiochem®
          SynonymsEpidermal Growth Factor
          References
          ReferencesCanals, F., et al. 1992. Biochemistry 31, 4493.
          Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
          Sato, J., et al. 1987. Methods Enzymol. 146, 63.
          Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.
          Product Information
          CAS number62229-50-9
          FormLyophilized
          Applications
          Biological Information
          Biological activitydetermined by mitogenic assay with human foreskin fibroblasts
          Purity≥98% by SDS-PAGE
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSKB4518000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324856

          References

          Reference overview
          Canals, F., et al. 1992. Biochemistry 31, 4493.
          Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
          Sato, J., et al. 1987. Methods Enzymol. 146, 63.
          Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-May-2008 RFH
          SynonymsEpidermal Growth Factor
          DescriptionNative epidermal growth factor from mouse submaxillary glands. Purified by size-exclusion chromatography. Exhibits mitogenic activity in a wide range of tissues of ectodermal and endodermal origin. Effective at concentrations ranging from 5-50 ng/ml.
          FormLyophilized
          CAS number62229-50-9
          RTECSKB4518000
          Purity≥98% by SDS-PAGE
          Biological activitydetermined by mitogenic assay with human foreskin fibroblasts
          SolubilityReconstitute in culture medium, buffer, H₂O, or 100 mM acetic acid. Wipe the vial with 70% ethanol, add solvent, and swirl gently to dissolve.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          Merck USA index14, 3527
          ReferencesCanals, F., et al. 1992. Biochemistry 31, 4493.
          Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
          Sato, J., et al. 1987. Methods Enzymol. 146, 63.
          Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.

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          Categories

          Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
          Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors