371209 | Dkk-2, Mouse, Recombinant

371209
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      371209-10UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 10 μg
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          Description
          OverviewRecombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag® sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
          Catalogue Number371209
          Brand Family Calbiochem®
          References
          ReferencesMorvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Mukhopadhyay, M. et al. 2006. Development 133, 2149.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Li, X. et al. 2005. Nat. Genet. 37, 945.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. and C. Niehrs 2003. Gene 302, 179.
          Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
          Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Wu, W. et al. 2000. Current Biol. 10,1611.
          Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
          Krupnik, V.E. et al. 1999. Gene 238, 301.
          Product Information
          FormSolid
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Applications
          Biological Information
          Biological activityInhibition assay:
          Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.

          Competetive Binding Assay:
          Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: <1.0 EU per/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          371209

          References

          Reference overview
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Mukhopadhyay, M. et al. 2006. Development 133, 2149.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Li, X. et al. 2005. Nat. Genet. 37, 945.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. and C. Niehrs 2003. Gene 302, 179.
          Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
          Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Wu, W. et al. 2000. Current Biol. 10,1611.
          Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
          Krupnik, V.E. et al. 1999. Gene 238, 301.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-September-2011 RFH
          DescriptionRecombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag® sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
          FormSolid
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: <1.0 EU per/µg protein
          Biological activityInhibition assay:
          Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.

          Competetive Binding Assay:
          Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
          SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesMorvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Mukhopadhyay, M. et al. 2006. Development 133, 2149.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Li, X. et al. 2005. Nat. Genet. 37, 945.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. and C. Niehrs 2003. Gene 302, 179.
          Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
          Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Wu, W. et al. 2000. Current Biol. 10,1611.
          Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
          Krupnik, V.E. et al. 1999. Gene 238, 301.