S7113 | DAPI/Antifade Solution, Ready to Use

S7113
1 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Detection Methods
      Fluorescent
      Description
      Catalogue NumberS7113
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDAPI/Antifade Solution, Ready to Use
      OverviewDAPI: 4', 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4μg/mL DAPI suspended in Antifade. The product is ready to add to fixed slides or cells cultures.
      References
      Product Information
      Detection methodFluorescent
      PresentationLiquid PBS pH 8.0 containing ~50% glycerol and 0.4μg/mL of DAPI and 2-7mM phenylenediamine.
      Applications
      Application NotesA nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, metaphase chromosomes, or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent stains. Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources.

      DAPI binds the minor groove of DNA at AT clusters and binds preferentially to ds DNA (Biochemistry 26:4545, 1987).

      Materials Provided

      Each vial contains 1 ml of DAPI/Antifade Solution

      Warnings and Precautions

      DAPI is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Use extreme caution. Wear gloves and a lab coat when handling. Follow your institution's guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.



      Related Products

      Antifade Solution S7114

      Propidium Iodide Solution S7109

      Propidium Iodide/Antifade Solution S7112
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20ºC in undiluted aliquots for up to 1 year from date of receipt. Protect from light
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      DAPI/Antifade Solution 2919599
      DAPI/Antifade Solution 3031654
      DAPI/Antifade Solution 3043678
      DAPI/Antifade Solution - 2120110 2120110
      DAPI/Antifade Solution - 2383145 2383145
      DAPI/Antifade Solution - 2004865 2004865
      DAPI/Antifade Solution - 2275576 2275576
      DAPI/Antifade Solution -2506512 2506512
      DAPI/Antifade Solution -2584394 2584394
      DAPI/Antifade Solution -2683875 2683875

      References

      Reference overviewPub Med ID
      Creation of Biofunctionalized Micropatterns on Poly(methyl methacrylate) by Single-Step Phase Separation Method.
      Ho QP, Wang SL, Wang MJ
      ACS applied materials & interfaces  3  4496-503. Epub 2011 Nov 3.  2011

      Show Abstract
      22022975 22022975
      Observation of chondrocyte aggregate formation and internal structure on micropatterned fibroin-coated surface.
      Naoyoshi D Kachi,Akihisa Otaka,Seungwoo Sim,Yoshihiko Kuwana,Yasushi Tamada,Junko Sunaga,Taiji Adachi,Naohide Tomita
      Bio-medical materials and engineering  20  2010

      Show Abstract
      20448304 20448304
      Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis.
      Yusuke Kambe,Koji Yamamoto,Katsura Kojima,Yasushi Tamada,Naohide Tomita
      Biomaterials  31  2010

      Show Abstract
      20643479 20643479

      Posters

      Title
      Dopamine Selectively Predisposes Dopaminergic Neurons In Primary Mesencephalic Cultures To Damage By An Oxidative Insult

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunohistochemistry (IHC) > Histological Stains and Staining Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunocytochemistry (ICC) > Immunocytochemistry Reagents
      Life Science Research > Cell Analysis > Cell-based Assays > Apoptosis / Cell Death Assays
      Life Science Research > Antibodies and Assays > Immunoassays > Immunohistochemistry (IHC) > Histological Stains and Staining Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunocytochemistry (ICC) > Immunocytochemistry Reagents