S7824 CpGenome Fast DNA Modification Kit

S7824
25 reactions  25 samples
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Catalogue NumberS7824
      Brand Family Chemicon®
      Trade Name
      • CpGenome
      • Chemicon
      DescriptionCpGenome Fast DNA Modification Kit
      OverviewThe CpGenome™ Fast DNA Modification Kit contains reagents required to perform a bisulfite modification on a DNA sample including DNA isolation columns for easy and efficient recovery of bisulfite treated DNA.
      Materials Required but Not DeliveredEquipment and Supplies

      a. Water bath incubator or heat block at 37°C and 50°C.

      b. Microcentrifuge (to 12,000X G)

      c. Screw-cap microcentrifuge tubes, 1.5-2.0 mL

      d. Narrow range pH indicator paper (pH 0-6, three different indicators per strip, and/or pH 4-7, one indicator per strip) or a dedicated pH electrode.

      Reagents

      a. NaOH pellets

      b. 100% EtOH

      c. Nuclease-free water
      Background InformationMethylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppresser genes in human cancers (7, 8). Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors.

      Several methods have been developed to determine the methylation status of cytosine. These include digestion with methylation sensitive restriction enzymes as in restriction landmark genomic scanning, oligonucleotide arrays, bisulfite genomic DNA sequencing and Methylation Specific PCR (MSP). Some techniques are more useful for discovery while others are better used for monitoring of known methylated cytosines. Genomic DNA sequencing, although time consuming and labor intensive, offers a more universal detection method. MSP is now an established technology for the monitoring of abnormal gene methylation in selected gene sequences. Utilizing small amounts of DNA, this procedure offers sensitive and specific detection of 5-methylcytosine in promoters. It is being exploited to define tumor suppresser gene function, and to provide a new strategy for early tumor detection.

      The initial step of both bisulfite genomic sequencing and MSP is to perform a bisulfite modification of the DNA sample. MSP then involves PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA. The CpGenome™ Fast DNA Modification Kit contains the reagents for the initial bisulfite modification of the DNA required for both methodologies.
      References
      Product Information
      Components
      • 90584 DNA Modification Reagent 6 g Room Temp
      • 90585 Binding Buffer 20 mL Room Temp
      • 90586 Wash Buffer* 12 mL Room Temp
      • 90587 Elution Buffer 1.5 mL Room Temp
      • 90588 DNA Modification Columns 25 Room Temp
      • Sufficient reagents are provided in the CpGenome™ Fast DNA Modification Kit to perform 25 bisulfite reactions.
      • * Wash Buffer requires the addition of 100% Ethanol.
      Applications
      ApplicationThe CpGenome Fast DNA Modification Kit contains the reagents for the initial bisulfite modification of the DNA required for both methodologies.
      Application NotesFor MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size25 reactions
      Material Package25 samples
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      CpGenome Fast DNA Modification Kit SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Epigenetic silencing of PTPRR activates MAPK signaling, promotes metastasis and serves as a biomarker of invasive cervical cancer.
      Su, PH; Lin, YW; Huang, RL; Liao, YP; Lee, HY; Wang, HC; Chao, TK; Chen, CK; Chan, MW; Chu, TY; Yu, MH; Lai, HC
      Oncogene  32  15-26  2013

      Show Abstract
      22330137 22330137
      Effects of perinatal hypothyroidism on regulation of reelin and brain-derived neurotrophic factor gene expression in rat hippocampus: Role of DNA methylation and histone acetylation.
      Sui L, Li BM
      Steroids  2010

      Show Abstract
      20600205 20600205
      Inactivation of O6-methylguanine-DNA methyltransferase in soft tissue sarcomas: association with K-ras mutations.
      Jeung Il Kim,Jeung Tak Suh,Kyung Un Choi,Hyun Jeong Kang,Dong Hoon Shin,In Sook Lee,Tae Yong Moon,Won Taek Kim
      Human pathology  40  2009

      Show Abstract
      19356788 19356788
      Expression of beta-catenin and its mechanism of delocalization in intestinal-type early gastric cancer based on mucin expression.
      S H Lee, H J Kang, D-H Shin, D-Y Cho, J M Song, H C Lee, G H Kim, G A Song, M Y Sol, J Y Kim, K U Choi, C H Lee, G Y Huh, D Y Park, Soo Han Lee, Hyun Jeong Kang, Dong-Hun Shin, Duk-Yeon Cho, Jin Mi Song, Han Cheol Lee, Gwang Ha Kim, Geun Am Song, Mee Young Sol, Jee Yeon Kim, Kyung Un Choi, Chang Hun Lee, Gi Young Huh, Do Youn Park
      Histology and histopathology  24  831-8  2009

      Show Abstract
      19475529 19475529

      Instructions for Use

      Title
      CpGenome¿ Fast DNA Modification Kit