17-10091 CpGenome™ 5-hmC Quantitation Kit

17-10091
96-well plate  Shipped in two separate boxes: Part No. 17-10091-1 containing 2°C to 8°C and room temperature storage components; Part No. 17-10091-2 containing -20°C storage components.
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      Overview

      Replacement Information
      Description
      Catalogue Number17-10091
      Trade Name
      • CpGenome
      • Upstate
      DescriptionCpGenome™ 5-hmC Quantitation Kit
      OverviewIdeal for global and tissue-specific detection and quantitation of 5-hmC in genomic DNA, as well as high-throughput screening of activators and inhibitors of 5-hmC modification.

      Features and Benefits
      • Compatible with genomic DNA from cultured cells or tissues
      • Includes set of 5-hmC of standards for precise quantitation
      • Streamlined, simple, automation-friendly ELISA-based protocol
      • Sensitive colorimetric detection with a limit of quantitation down to 0.03% of input DNA.
      Materials Required but Not Delivered
      • Incubator, thermal cyclers
      • Plate reader capable of 405 nm detection
      • Multichannel pipettor (recommended)
      • Parafilm®, Pipette tips, dilution tubes, and other assorted consumables
      Background InformationRapidly quantify levels of 5-hmC DNA in a wide range of DNA samples with a limit of detection down to 0.03% of input DNA.
      The CpGenome™ 5-hmC Quantitation Kit enables determination of relative and absolute levels of 5-hydroxymethyl cytosine (5-hmC) in genomic DNA samples. This assay utilizes a rapid, streamlined, automation-compatible, ELISA-based protocol for sensitive quantification of 5-hmC DNA obtained from cultured cells and tissues. With quantitation based on a set of precisely calibrated standards, this assay is ideal for experiments where global and/or tissue-specific detection and quantitation of 5-hmC DNA is required.
      References
      Product Information
      Components
      • Plate Coating Buffer (15 mL)
        10X Assay Buffer (30 mL)
        HRP Detection Reagent (15 mL)
        96-well Assay Plate and 8-well Strip Caps (1 plate/12 strips)
        5-hmC Antibody (1mg/mL)
        100x HRP Detection Antibody (1 mg/mL)
        5-hmC DNA Quantitation Standards - 5 vials for creation of standard curve (100 ng/µL each)
      PresentationTwo boxes containing all necessary reagents to perform quantitation of 5-hmC levels in a 96-well plate.
      Applications
      ApplicationThe CpGenome 5-hmC Quantitation Kit enables determination of relative & absolute levels of 5-hydroxymethyl cytosine (5-hmC) in genomic DNA samples.
      Biological Information
      Modifications
      • Hydroxylation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 month year from date of shipment when stored and handled as directed.
      Packaging Information
      Material Size96-well plate
      Material PackageShipped in two separate boxes: Part No. 17-10091-1 containing 2°C to 8°C and room temperature storage components; Part No. 17-10091-2 containing -20°C storage components.
      Transport Information
      Supplemental Information
      Specifications