204886 | Complement C4, Human

204886
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      204886-250UG
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          Plastic ampoule 250 μg
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          Description
          OverviewNative, human C4 complement component. Glycoprotein composed of three non-identical subunits of M.W. of 93 kDa (α), 75 kDa (β), and 32 kDa (γ) linked by disulfide bonds. Present in normal human serum at 400 µg/ml. On activation of complement via the classical pathway, the C1s subcomponent of the C1 complex is converted to an active serine protease that cleaves the C4 α-chain at peptide bond 77, resulting in production of C4a (M.W. 8740) and C4b fragments (M.W. 193 kDa). The released C4a peptide is one of the three complement-derived anaphylatoxins. The nascent C4b fragment can form a covalent ester bond with target surfaces. This covalent attachment of C4b to target acceptors is required for continuation of activation via classical pathway.
          Catalogue Number204886
          Brand Family Calbiochem®
          References
          ReferencesJanatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
          Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.
          Product Information
          CAS number80295-48-3
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Applications
          Biological Information
          Biological activity≥60% of the C4 activity present in freshly drawn normal human serum on a mg to mg basis
          Purity≥90% by SDS-PAGE
          SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          Specific Activity>3X10⁵ C4H50 units/mg
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          204886

          References

          Reference overview
          Janatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
          Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.

          Citations

          Title
        • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-May-2008 RFH
          DescriptionNative, human C4 complement component. Glycoprotein composed of three non-identical subunits of M.W. 93,000 (α), 75,000 (β), and 32,000 (γ) linked by disulfide bonds. Present in normal human serum at 400 µg/ml. On activation of complement via the classical pathway, the C1s subcomponent of the C1 complex is converted to an active serine protease that cleaves the C4 α-chain at peptide bond 77, resulting in the production of C4a (M.W. 8740) and C4b fragments (M.W. 193,000). The released C4a peptide is one of the three complement-derived anaphylatoxins. The nascent C4b fragment can form a covalent ester bond with target surfaces. This covalent attachment of C4b to target acceptors is required for continuation of activation via classical pathway.
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Concentration Label Please refer to vial label for lot-specific concentration
          SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          CAS number80295-48-3
          Purity≥90% by SDS-PAGE
          Specific activity>3X10⁵ C4H50 units/mg
          Biological activity≥60% of the C4 activity present in freshly drawn normal human serum on a mg to mg basis
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesJanatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
          Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.
          Citation
        • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.