204885 Complement C3, Human

204885
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      204885-250UG
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          Plastic ampoule 250 μg
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          Description
          OverviewNative, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115 kDa (α) and 75 kDa (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77 resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. This covalent attachment of C3b to target acceptors is required for continuation of complement activation.
          Catalogue Number204885
          Brand Family Calbiochem®
          References
          ReferencesLambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol. 23, 1237.
          Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.
          Product Information
          CAS number80295-41-6
          FormLiquid
          FormulationIn PBS.
          Applications
          Biological Information
          Biological activity>3,000 C₃H₅0 units/mg; ≥60% of the C3 activity in normal human serum on a mg/mg basis.
          Purity≥85% by SDS-PAGE
          SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          Physicochemical Information
          ContaminantsIgG, IgA, IgM, C4, functionally active C5, albumin or ceruloplasmin: ≤trace amounts
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          204885

          References

          Reference overview
          Lambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol. 23, 1237.
          Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.

          Citations

          Title
        • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision29-June-2018 JSW
          DescriptionNative, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115,000 (α) and 75,000 (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at the C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77, resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. The covalent attachment of C3b to target acceptors is required for continuation of complement activation.
          FormLiquid
          FormulationIn PBS.
          SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          CAS number80295-41-6
          Purity≥85% by SDS-PAGE
          ContaminantsIgG, IgA, IgM, C4, functionally active C5, albumin or ceruloplasmin: ≤trace amounts
          Biological activity>3,000 C₃H₅0 units/mg; ≥60% of the C3 activity in normal human serum on a mg/mg basis.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesLambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol. 23, 1237.
          Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.
          Citation
        • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.