234153 Collagenase, Type I, Clostridium histolyticum

234153
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      234153-1GM
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          Plastic ampoule 1 gm
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          Description
          Overview

          This product has been discontinued.



          Crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies.

          Note: 1 ABC unit = 1.1 BTC unit.
          Catalogue Number234153
          Brand Family Calbiochem®
          References
          ReferencesHarris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
          Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.
          Product Information
          CAS number9001-12-1
          Activity≥125 ABC units/mg solid
          Unit of DefinitionOne ABC unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent per min from collagen at 37°C, pH 7.2. Note: 1 ABC unit = 1.1 BTC (bovine tendon collagen) unit.
          EC number3.4.24.3
          FormLyophilized
          Hygroscopic Hygroscopic
          Applications
          Biological Information
          Physicochemical Information
          ContaminantsCaseinase activity: ≤100 FFC unit/500 ABC unit
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38-42/43

          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation and skin contact.
          S PhraseS: 22-24-37

          Do not breathe dust.
          Avoid contact with skin.
          Wear suitable gloves.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Collagenase, Type I, Clostridium histolyticum SDS

          Title

          Safety Data Sheet (SDS) 

          Collagenase, Type I, Clostridium histolyticum Certificates of Analysis

          TitleLot Number
          234153

          References

          Reference overview
          Harris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
          Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-February-2017 JSW
          DescriptionCrude enzyme preparation that contains several collagenases, a sulfhydryl protease, clostripain and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies. Most active on fat cells, adrenal and liver tissue, with an optimal pH of 7.0-8.0.
          FormLyophilized
          CAS number9001-12-1
          EC number3.4.24.3
          ContaminantsCaseinase activity: ≤100 FFC unit/500 ABC unit
          Activity≥125 ABC units/mg solid
          Unit definitionOne ABC unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent per min from collagen at 37°C, pH 7.2. Note: 1 ABC unit = 1.1 BTC (bovine tendon collagen) unit.
          SolubilityAqueous buffers (1 mg/ml)
          Storage +2°C to +8°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
          Toxicity Harmful
          Merck USA index14, 2481
          ReferencesHarris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med. 291, 557.
          Seifter, S., and Harper, E. 1970. Methods Enzymol. 19, 613.