234149 | Collagen, Type I, Human Placenta

234149
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      234149-250UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 250 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewHighly purified, native, type I collagen from human placenta. Useful as a gel or thin coating for the attachment of cells. Recommended concentration: 0.2 - 2 µg/cm2 of growth surface.
          Catalogue Number234149
          Brand Family Calbiochem®
          References
          ReferencesBosco, M., et al. 1993. J. Cell Biol. 120, 537.
          Leavesley, D.I., et al. 1993. J. Cell Biol. 121, 163.
          Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys. 301, 103.
          Streuli, C.H., et al. 1993. J. Cell Biol. 120, 253.
          Karsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
          Klasson, S.C., et al. 1986. Coll. Relat. Res. 6, 397.
          Product Information
          CAS number9007-34-5
          FormLiquid
          FormulationIn 10 mM HCl.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          SourcePrepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          234149

          References

          Reference overview
          Bosco, M., et al. 1993. J. Cell Biol. 120, 537.
          Leavesley, D.I., et al. 1993. J. Cell Biol. 121, 163.
          Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys. 301, 103.
          Streuli, C.H., et al. 1993. J. Cell Biol. 120, 253.
          Karsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
          Klasson, S.C., et al. 1986. Coll. Relat. Res. 6, 397.

          Citations

          Title
        • Emily K. Thompson, et al. (2005) Endo180 binds to the C-terminal region of type I collagen. Journal of Biological Chemistry 280, 22596-22605.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision18-June-2010 JSW
          DescriptionHighly purified, native, type I collagen from human placenta. Useful as a gel or thin coating for the attachment of cells. Facilitates in vitro cultivation of cells and enhances cell-specific morphology and function. The recommended concentration is 0.2-2 µg/cm2 of growth surface.
          FormLiquid
          FormulationIn 10 mM HCl.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          Coating Procedure Guidelines
          Note: The optimal concentration for cell attachment and culture may differ for different cell types, and experimentation may be required to determine the optimal conditions for your cell culture system.
          Human Collagen, Type I is typically used as a thin coating on plastic tissue culture plates. Alternatively, it may also be gelled.
          Guidelines for Coating Tissue Culture Plates (Thin Coating)
          1. Add sufficient volume of collagen to provide desired coating concentration. A coating concentration of 0.2 µg/cm2 to 2 µg/cm2 is recommended, depending on the cell type. It is important that the volume added to the dish is sufficient to cover the growth surface. If necessary, dilute the collagen stock with 2 mM HCl.
          2. Cover the growth surface completely covered and incubate for 2 h at room temperature.
          3. Tilt the dish just enough to allow excess collagen to drain to the lowest point in dish. Remove excess material with sterile pipette.
          4. Air dry the plate by leaving the lid slightly ajar in a laminar flow tissue culture hood or dry with a gentle stream of sterile gas.
          5. Once dry, the plates are ready for use.
          Guidelines for Gelling
          1. Dilute the collagen to desired concentration using 2 mM HCl.
          2. Mix 9 parts Collagen, Type I, Human Placenta and 1 part 10X buffer or 10X media.
          3. Add the mixture to the desired tissue culture vessel.
          4. Incubate for 15 to 60 min at 37°C. Gel is then ready for use, but must be handled carefully.
          SourcePrepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          CAS number9007-34-5
          Purity≥90% by SDS-PAGE
          SolubilityFurther dilute with acetic acid (10 mM)
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          Merck USA index14, 2480
          ReferencesBosco, M., et al. 1993. J. Cell Biol. 120, 537.
          Leavesley, D.I., et al. 1993. J. Cell Biol. 121, 163.
          Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys. 301, 103.
          Streuli, C.H., et al. 1993. J. Cell Biol. 120, 253.
          Karsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
          Klasson, S.C., et al. 1986. Coll. Relat. Res. 6, 397.
          Citation
        • Emily K. Thompson, et al. (2005) Endo180 binds to the C-terminal region of type I collagen. Journal of Biological Chemistry 280, 22596-22605.