70573 Clonables™ 2X Ligation Premix - Novagen

70573
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      70573-3
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          Plastic ampoule 55 ul
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          70573-4
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              Plastic ampoule 2.5 ml
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              Description
              OverviewThe Clonables™ 2X Ligation Premix is a single solution containing optimized concentrations of the highest quality T4 DNA ligase, buffer, stabilizer, and cofactors needed for efficient ligation of any type of compatible DNA ends. The premix is tested for ligation of compatible 2- to 4-base cohesive DNA ends as well as blunt ends and single-base overhangs found on some PCR products. Under most conditions excellent ligation is achieved in only 15 minutes; with T/A or U/A overhangs more colonies can be obtained by incubating up to two hours. Although maximal efficiencies are obtained using NovaBlue Singles Competent Cells, this reagent is compatible with transformation of any type of chemically competent cells provided by the user. The high performance, minimal pipetting requirement, and single-addition format make the premix suitable for high-throughput applications.

              One vial of the 2X Ligation Premix (Cat. No. 70573-3) contains enough reagent for 11 ligation reactions (10-µl scale).
              Catalogue Number70573
              Brand Family Novagen®
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              Ship Code Shipped with Blue Ice or with Dry Ice
              Toxicity Irritant
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
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