CYT304 ChemiKine Nerve Growth Factor, Sandwich ELISA

CYT304
1 kit  192 wells
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      NGF M, R ELISA Chromogenic
      Description
      Catalogue NumberCYT304
      Brand Family Chemicon®
      Trade Name
      • ChemiKine
      • Chemicon
      DescriptionChemiKine Nerve Growth Factor, Sandwich ELISA
      OverviewWith the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample.

      NGF specific mouse monoclonal antibodies detect the captured NGF. Mouse specific donkey polyclonal antibodies, peroxidase labeled, detect the mouse monoclonal antibody. After addition of the substrate and stop solution the amount of NGF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and NGF concentration: i.e., the higher the OD the higher the NGF concentration in the sample.ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. The kit utilizes strip well inserts. Running duplicate wells for samples and standards is recommended.

      Analytical Sensitivity and Detection Limits:

      Sensitivity:10-15 pg/mL

      Range of Detection: 10 pg/mL to 500 pg/mL

      Crossreactivity: No significant cross reactivity with BDNF, NT4/5 or NT3

      Intra-assay Variation:± 4.1% (250 pg/mL)

      Inter-assay Variation:± 8.9 % (250 pg/mL)

      Test Principle:

      With the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample. NGF specific mouse monoclonal antibodies detect the captured NGF. Mouse specific donkey polyclonal antibodies, peroxidase labeled, detect the mouse monoclonal antibody. After addition of the substrate and stop solution the amount of NGF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and NGF concentration: i.e., the higher the OD the higher the NGF concentration in the sample.

      Application:

      ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

      For Research Use Only. Not for use in diagnostic procedures.
      Alternate Names
      • NGF
      Materials Required but Not Delivered1. Multi-channel or repeating pipettes

      2. Plate shaker (optional)

      3. Pipettors & tips capable of accurately measuring 10-1000 mL

      4. Graduated serological pipettes

      5. 96-well microplate Reader with 450 nm filter

      6. Graph paper for manual plotting of data

      7. Polystyrene test tubes for standard and sample dilutions

      8. Mechanical vortex

      9. One 1 or 2 liter container
      References
      Product Information
      Components
      • ChemiKine NGF ELISA Plate: (Part No. 90139) Two Pre-coated with Sheep anti-Mouse NGF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.
      • Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.
      • Assay Diluent: (Part No. 90143) One 100 mL bottle (Ready to Use).
      • NGF Standard (Mouse): (Part No. 90142) Two vials (Lyophilized).
      • Mouse anti-Mouse NGF Monoclonal Antibody: (Part No. 90141) Two vials (Lyophilized).
      • Donkey anti-Mouse IgG Polyclonal Antibody, HRP conjugated: (Part No. 90140) Two vials (Lyophilized).
      • TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.
      • Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution (Ready to Use).
      Detection methodChromogenic
      Applications
      ApplicationThis ChemiKine Nerve Growth Factor, Sandwich ELISA is used to measure & quantify NGF levels in Neuroscience research.
      Key Applications
      • ELISA
      Biological Information
      Species Reactivity
      • Mouse
      • Rat
      Analytes Available
      • NGF
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
      Gene Symbol
      • NGF
      • NGFB
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Storage ConditionsPrecautions:

      · Wash Buffer and Assay Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

      · The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.
      Packaging Information
      Material Size1 kit
      Material Package192 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ChemiKine Nerve Growth Factor, Sandwich ELISA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Synergistic effects of granulocyte-colony stimulating factor on bone marrow stromal cell transplantation for mice cerebral infarct.
      Masaaki Hokari,Satoshi Kuroda,Yasuhiro Chiba,Katsuhiko Maruichi,Yoshinobu Iwasaki
      Cytokine  46  2009

      Show Abstract
      19286390 19286390
      Bone marrow stromal cells protect and repair damaged neurons through multiple mechanisms.
      Masaaki Hokari,Satoshi Kuroda,Hideo Shichinohe,Shunsuke Yano,Kazutoshi Hida,Yoshinobu Iwasaki
      Journal of neuroscience research  86  2008

      Show Abstract
      18030676 18030676
      Peripheral levels of BDNF and NGF in primary headaches.
      F Blandini, L Rinaldi, C Tassorelli, G Sances, M Motta, A Samuele, R Fancellu, G Nappi, A Leon
      Cephalalgia : an international journal of headache  26  136-42  2006

      Show Abstract
      16426267 16426267
      Microglia-Müller glia cell interactions control neurotrophic factor production during light-induced retinal degeneration.
      Harada, Takayuki, et al.
      J. Neurosci., 22: 9228-36 (2002)  2002

      Show Abstract
      12417648 12417648

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