219467 Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells

219467
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      219467-10UG
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.
          Catalogue Number219467
          Brand Family Calbiochem®
          SynonymsCathepsin L2, Cathepsin U
          References
          ReferencesTolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
          Turk, V., et al. 2001. EMBO J. 20, 4629.
          Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
          Bromme, D., et al. 1999. Biochemistry 38, 2377.
          Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
          Santamaria, I., et al. 1998. Cancer Res. 58, 1624.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
          EC number3.4.22.43
          FormFrozen liquid
          FormulationIn 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.
          Applications
          Biological Information
          Purity≥95% by SDS-PAGE
          Specific Activity≥300 units/µg
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          ContaminantsEndotoxins: ≤1.0 EU/µg Cathepsin V
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsTo activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          219467

          References

          Reference overview
          Tolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
          Turk, V., et al. 2001. EMBO J. 20, 4629.
          Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
          Bromme, D., et al. 1999. Biochemistry 38, 2377.
          Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
          Santamaria, I., et al. 1998. Cancer Res. 58, 1624.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-November-2008 RFH
          SynonymsCathepsin L2, Cathepsin U
          DescriptionRecombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.
          FormFrozen liquid
          FormulationIn 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          EC number3.4.22.43
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxins: ≤1.0 EU/µg Cathepsin V
          Specific activity≥300 units/µg
          Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsTo activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesTolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
          Turk, V., et al. 2001. EMBO J. 20, 4629.
          Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
          Bromme, D., et al. 1999. Biochemistry 38, 2377.
          Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
          Santamaria, I., et al. 1998. Cancer Res. 58, 1624.