218809 Caspase Substrate Set IV, Fluorogenic - Calbiochem

218809
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      218809-1SET
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          Glass bottle 1 set
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          Description
          OverviewReady-to-use substrates for assaying the activity of various members of the caspase-family proteases. Contains 125 µl (1 mM) each of Caspase-1 Substrate VI, Ac-YVAD-AFC (Cat. No. 688224); Caspase-2 Substrate I, Ac-VDVAD-AFC (Cat. No. 218740); Caspase-3 Substrate IV, Ac-DEVD-AFC (Cat. No. 264150); Caspase-5 Substrate II, Ac-WEHD-AFC (Cat. No. 218754); Caspase-6 Substrate III, Ac-VEID-AFC (Cat. No. 218763); Caspase-8 Substrate I, Z-IETD-AFC (Granzyme B Substrate II, Cat. No. 368059); and Caspase-9 Substrate I, Ac-LEHD-AFC (Cat. No. 218765). Supplied with a data sheet.
          Catalogue Number218809
          Brand Family Calbiochem®
          Application Data
          References
          ReferencesHumke, E.W., et al. 1998. J. Biol. Chem. 273, 15702.
          Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
          Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123.
          Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
          Nicholson, D.W., et al. 1995. Nature 376, 37.
          Thornberry, N.A., et al. 1992. Nature 356, 768.
          Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.
          Product Information
          FormLiquid
          FormulationEach substrate supplied at 1 mM in DMSO.
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/38

          Irritating to eyes and skin.
          S PhraseS: 26

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          218809

          References

          Reference overview
          Humke, E.W., et al. 1998. J. Biol. Chem. 273, 15702.
          Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
          Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123.
          Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
          Nicholson, D.W., et al. 1995. Nature 376, 37.
          Thornberry, N.A., et al. 1992. Nature 356, 768.
          Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-September-2014 JSW
          Application Data
          DescriptionApoptosis is a normal process in development and morphogenesis. However, improperly regulated apoptosis can contribute to several pathological conditions including cancer, Alzheimer's disease, spinal muscular atrophy, ischemic cardiac damage, and autoimmune syndromes. Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. Receptor-mediated apoptosis involves the catalysis of preexisting intracellular caspase proenzymes into activated caspases (cysteine-containing aspartate-specific proteases). A distinctive feature of caspases is the requirement of an aspartic acid residue in the substrate P1 position. The P4 residue is important in substrate recognition and specificity. For example, caspase-1 prefers a hydrophobic tyrosine residue in the P4 position, but caspase-3 prefers an anionic aspartic residue. Caspase activity can be assayed using a fluorophore (AFC). Liberation of the fluorophore from the substrate is monitored to determine caspase activity. Supplied with a data sheet.

          This convenient set of caspase substrates contains the following components, each at a concentration of 1 mM in DMSO:
          FormLiquid
          FormulationEach substrate supplied at 1 mM in DMSO.
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Irritant
          ReferencesHumke, E.W., et al. 1998. J. Biol. Chem. 273, 15702.
          Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
          Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123.
          Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
          Nicholson, D.W., et al. 1995. Nature 376, 37.
          Thornberry, N.A., et al. 1992. Nature 356, 768.
          Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.