218785 | Caspase-10, Human, Recombinant, E. coli

218785
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      218785-3000U
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 3000 u
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          Description
          OverviewRecombinant, human caspase-10 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
          M.W. 11,000 and 18,000 (heterodimer).
          Catalogue Number218785
          Brand Family Calbiochem®
          SynonymsMch4, FLICE-2
          References
          ReferencesKang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
          Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
          Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
          Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
          Product Information
          Activity≥50,000 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=368057">Cat. No. 368057</a>) per min at 25°C, pH 7.4.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.
          Applications
          Biological Information
          Purity≥95% by SDS-PAGE
          Specific Activity≥150,000 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          218785

          References

          Reference overview
          Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
          Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
          Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
          Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-September-2007 RFH
          SynonymsMch4, FLICE-2
          DescriptionRecombinant, human caspase-10 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.
          Purity≥95% by SDS-PAGE
          Specific activity≥150,000 units/mg protein
          Activity≥50,000 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesKang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
          Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
          Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
          Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.