382061 Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem

382061
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₅H₂₄N₆O · 3HCl 23491-45-4

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      382061-100MG
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          Plastic ampoule 100 mg
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          382061-1GM
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              Plastic ampoule 1 gm
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              382061-250MG
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                  Plastic ampoule 250 mg
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                  Description
                  OverviewMembrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.
                  Catalogue Number382061
                  Brand Family Calbiochem®
                  Synonyms2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
                  References
                  ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
                  Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
                  Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
                  Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
                  Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
                  Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
                  Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
                  Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
                  Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
                  Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.
                  Product Information
                  CAS number23491-45-4
                  ATP CompetitiveN
                  FormYellow-green solid
                  Hill FormulaC₂₅H₂₄N₆O · 3HCl
                  Chemical formulaC₂₅H₂₄N₆O · 3HCl
                  ReversibleN
                  Structure formula ImageStructure formula Image
                  Applications
                  Biological Information
                  Primary TargetAdenine-thymine-specific fluorescent stain
                  Purity≥98% by HPLC
                  Physicochemical Information
                  Cell permeableY
                  Emission max.
                  Excitation max.
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  RTECSSM1140500
                  Safety Information
                  R PhraseR: 36/37/38-40

                  Irritating to eyes, respiratory system and skin.
                  Limited evidence of a carcinogenic effect.
                  S PhraseS: 22-26-36

                  Do not breathe dust.
                  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                  Wear suitable protective clothing.
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Carcinogenic / Teratogenic
                  Storage +2°C to +8°C
                  Protect from Light Protect from light
                  Do not freeze Ok to freeze
                  Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Certificates of Analysis

                  TitleLot Number
                  382061

                  References

                  Reference overview
                  Werstuck, G., and Green, M.R. 1998. Science 282, 296.
                  Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
                  Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
                  Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
                  Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
                  Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
                  Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
                  Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
                  Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
                  Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.

                  Brochure

                  Title
                  Dyes and Stains Technical Bulletin
                  Data Sheet

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision07-June-2015 JSW
                  Synonyms2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
                  DescriptionMembrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5' untranslated region (UTR) to control expression of transfected gene constructs.
                  FormYellow-green solid
                  CAS number23491-45-4
                  RTECSSM1140500
                  Chemical formulaC₂₅H₂₄N₆O · 3HCl
                  Structure formulaStructure formula
                  Purity≥98% by HPLC
                  SolubilityH₂O (20 mg/ml). Precipitates in phosphate buffered solutions
                  Storage +2°C to +8°C
                  Protect from light
                  Do Not Freeze Ok to freeze
                  Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.
                  Toxicity Carcinogenic / Teratogenic
                  ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
                  Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
                  Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
                  Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
                  Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
                  Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
                  Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
                  Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
                  Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
                  Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.

                  Related Products & Applications

                  Alternative Products

                  Catalog Number Description  
                  382065 Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem Show Pricing & Availability

                  Categories

                  Life Science Research > Inhibitors and Biochemicals > Biochemicals > Dyes, Stains and Probes > DNA-Binding Dyes