343222 Biotin, Fluorescein Conjugate

343222
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      343222-1MG
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          Plastic ampoule 1 mg
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          Description
          Catalogue Number343222
          Brand Family Calbiochem®
          References
          ReferencesGruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203
          Product Information
          FormLyophilized orange solid
          Applications
          Key Applications Enzyme Immunoassay
          Immunohistochemistry
          Application NotesEIA (1:1,000)
          Tissue Staining (1:50 - 1:100)
          Application CommentsMake a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          343222

          References

          Reference overview
          Gruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-September-2007 RFH
          ApplicationEIA (1:1,000)
          Tissue Staining (1:50 - 1:100)
          DescriptionAvidin (and streptavidin) has 4 binding sites for biotin. Taking advantage of the high affinity between avidin and biotin, this fluorescein-conjugated biotin-X (a 6-atom spacer) provides a method for fluorescent detection of biotinylated antibodies.
          FormLyophilized orange solid
          Recommended reaction conditionsFor tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50-1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1000.
          SolubilityDMF (5 mg/ml), DMSO (5 mg/ml), or Methanol (5 mg/ml)
          CommentsMake a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesGruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203