203639 BMP-4, Mouse, Recombinant

203639
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      203639-10UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 10 μg
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          Description
          OverviewRecombinant, mouse BMP-4 consisting of amino acids 293-408 and expressed in Chinese Hamster Ovary cells. BMP-4 plays a key role in epidermal determination, the development of multiple organs, suppression of neural induction, tissue repair, and mesenchyme formation.
          Catalogue Number203639
          Brand Family Calbiochem®
          References
          ReferencesLavery, K., et al. 2008. J. Biol. Chem. April 24 epub.
          Zhang, P., et al. 2008. Blood 111, 1933.
          Gambaro, K., et al. 2006. Cell Death Differ. 13, 1075.
          Rosen, V., 2006. Ann. N.Y. Acad. Sci. 1068, 19.
          Frank, D.B., et al. 2005. Circ. Res. 97, 496.
          Simic, P. and S. Vukicevic. 2005. Cytokine Growth Factor Rev. 16, 299.
          Chen, D., et al. 2004. Growth Factors 22, 233.
          Sadlon, T.J., et al. 2004. Stem Cells 22 457.
          Takada, T., et al. 2003. J. Biol. Chem. 278 43229.
          Cui, Y., et al. 2001. Genes Dev. 15, 2797.
          Cui, Y., et al. 1998. EMBO J. 17, 4735.
          Jones, C.M. and J.C. Smith. 1998. Dev. Biol. 194, 12.
          Nishimatsu, S. and G.H. Thomsen. 1998. Mech. Dev. 74 75.
          Aono, A. et al. 1995. Biochem. Biophys. Res. Commun. 210, 670.
          Oida, S., et al. 1995. DNA Seq. 5, 273.
          Product Information
          FormLiquid
          FormulationLyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA, BSA.
          Applications
          Biological Information
          Biological activityED₅₀ = 10-40 ng/ml as measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: <1.0 EU/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          203639

          References

          Reference overview
          Lavery, K., et al. 2008. J. Biol. Chem. April 24 epub.
          Zhang, P., et al. 2008. Blood 111, 1933.
          Gambaro, K., et al. 2006. Cell Death Differ. 13, 1075.
          Rosen, V., 2006. Ann. N.Y. Acad. Sci. 1068, 19.
          Frank, D.B., et al. 2005. Circ. Res. 97, 496.
          Simic, P. and S. Vukicevic. 2005. Cytokine Growth Factor Rev. 16, 299.
          Chen, D., et al. 2004. Growth Factors 22, 233.
          Sadlon, T.J., et al. 2004. Stem Cells 22 457.
          Takada, T., et al. 2003. J. Biol. Chem. 278 43229.
          Cui, Y., et al. 2001. Genes Dev. 15, 2797.
          Cui, Y., et al. 1998. EMBO J. 17, 4735.
          Jones, C.M. and J.C. Smith. 1998. Dev. Biol. 194, 12.
          Nishimatsu, S. and G.H. Thomsen. 1998. Mech. Dev. 74 75.
          Aono, A. et al. 1995. Biochem. Biophys. Res. Commun. 210, 670.
          Oida, S., et al. 1995. DNA Seq. 5, 273.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-Sepmtember-2011 RFH
          DescriptionRecombinant, mouse BMP-4 consisting of amino acids 293-408 and expressed in Chinese Hamster Ovary cells. BMP-4 plays a key role in epidermal determination, the development of multiple organs, suppression of neural induction, tissue repair, and mesenchyme formation.
          FormLiquid
          FormulationLyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA, BSA.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: <1.0 EU/µg protein
          Biological activityED₅₀ = 10-40 ng/ml as measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
          SolubilityReconstitute to 100 µg/ml in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesLavery, K., et al. 2008. J. Biol. Chem. April 24 epub.
          Zhang, P., et al. 2008. Blood 111, 1933.
          Gambaro, K., et al. 2006. Cell Death Differ. 13, 1075.
          Rosen, V., 2006. Ann. N.Y. Acad. Sci. 1068, 19.
          Frank, D.B., et al. 2005. Circ. Res. 97, 496.
          Simic, P. and S. Vukicevic. 2005. Cytokine Growth Factor Rev. 16, 299.
          Chen, D., et al. 2004. Growth Factors 22, 233.
          Sadlon, T.J., et al. 2004. Stem Cells 22 457.
          Takada, T., et al. 2003. J. Biol. Chem. 278 43229.
          Cui, Y., et al. 2001. Genes Dev. 15, 2797.
          Cui, Y., et al. 1998. EMBO J. 17, 4735.
          Jones, C.M. and J.C. Smith. 1998. Dev. Biol. 194, 12.
          Nishimatsu, S. and G.H. Thomsen. 1998. Mech. Dev. 74 75.
          Aono, A. et al. 1995. Biochem. Biophys. Res. Commun. 210, 670.
          Oida, S., et al. 1995. DNA Seq. 5, 273.