S7115 | ApopTag® Positive Control Slides, rat mammary gland 4d post weaning

S7115
5 ea  
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      Overview

      Replacement Information

      Key Specifications Table

      Description
      Catalogue NumberS7115
      Brand Family Chemicon®
      Trade Name
      • ApopTag
      • Chemicon
      DescriptionApopTag® Positive Control Slides, rat mammary gland 4d post weaning
      OverviewThis product is intended as a positive control for use with all ApopTag® In Situ Apoptosis Detection Kits. These reference slides are also useful as teaching aides, and for procedural troubleshooting. Each pack of ApopTag® Control Slides contains 5 unstained positive control slides. Rat mammary glands were obtained at the fourth day after weaning and were fixed for 18 hours in 10% neutral buffered formalin. After embedding in paraffin, 5 μm thick sections were cut from the middle of the tissue and were mounted on silanized slides.
      Background InformationRat mammary gland undergoes programmed cell death processes during the tissue regression that follows lactation. Apoptotic bodies, which can be seen by routine histologic staining, can be specifically stained by techniques that localize DNA strand breaks in situ. The specificity of staining for strand breaks can thus be correlated with the morphological changes that define the process of apoptosis.
      References
      Product Information
      Applications
      ApplicationThis product is intended as a positive control for use with all ApopTag In Situ Apoptosis Detection Kits.
      Application NotesInterpretation of Staining

      These positive control slides of female rat mammary tissue 4-days post weaning show only 1-2% of the cells being apoptotic. Microscopic inspection is usually required to confirm proper kit activity.

      Stain is generally localized to chromatin, and is intensely colored. The localization and sensitivity to be expected with the ApopTag® Peroxidase (S7100, S7101) or ApopTag® Fluorescein (S7110, S7111, S7160, S7165) Kits are similar. Positive objects vary in size, from intact nuclei to much smaller apoptotic bodies, and in shape, from round to irregular. Such objects may be either isolated, clustered, or engulfed in the cytoplasm of a phagocytic cell. Some unstained apoptotic nuclei or bodies may also be expected. Light cytoplasmic staining might co-localize with intense chromatin staining (this might be due to fragmented DNA leaching from the nucleus, or that in phagocytic organelles). If staining of non-apoptotic cell nuclei is observed with the ApopTag® Peroxidase kits (S7100, S7101), the user can attempt to decrease the substrate development time or temperature, or to further dilute the anti-digoxigenin antibody reagent in 0.5% (w:v) BSA. Counterstaining is recommended (see The Complete ApopTag® Manual).



      Limitations

      This control tissue has been selected for positive staining with all ApopTag® Kits. Results with control tissue may vary from results with other tissues because of both sample preparation differences and intrinsic tissue differences.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceApopTag® Control Slides have been qualified for use with all ApopTag® Kits.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended Storage 18°C to 25°C.
      Packaging Information
      Material Size5 ea
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      APOPTAG#174; Positive Control Slide - 2202524 2202524
      APOPTAG® Positive Control Slide 2926182
      APOPTAG® Positive Control Slide 3019055
      APOPTAG® Positive Control Slide - 2430542 2430542
      APOPTAG® Positive Control Slide - 2293849 2293849
      APOPTAG® Positive Control Slide - 2510333 2510333
      APOPTAG® Positive Control Slide -2701041 2701041
      APOPTAG® Positive Control Slide -2750280 2750280
      APOPTAG® Positive Control Slide -2794916 2794916

      References

      Reference overviewPub Med ID
      Local exposure of 849 MHz and 1763 MHz radiofrequency radiation to mouse heads does not induce cell death or cell proliferation in brain.
      Kim, TH; Kim, TH; Huang, TQ; Jang, JJ; Kim, MH; Kim, HJ; Lee, JS; Pack, JK; Seo, JS; Park, WY
      Experimental & molecular medicine  40  294-303  2008

      Show Abstract Full Text Article
      18587267 18587267