ABN1383 Anti-phospho-TrkA (Tyr794) Antibody

ABN1383
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H WB, ICC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABN1383
      DescriptionAnti-phospho-TrkA (Tyr794) Antibody
      Alternate Names
      • High affinity nerve growth factor receptor
      • Neurotrophic tyrosine kinase receptor type 1
      • Slow nerve growth factor receptor
      • p140-TrkA
      • Trk-A
      • phospho-TrkA (Tyr794)
      Background InformationHigh affinity nerve growth factor receptor (EC 2.7.10.1; UniProt P35739; also known as Neurotrophic tyrosine kinase receptor type 1, p140-TrkA, Slow nerve growth factor receptor, Trk-A, TrkA neurotrophin receptor, TrkA proto-oncogene receptor, Tropomyosin-related kinase A) is encoded by the Ntrk1 gene (also known as Trk, Trka) in rat (Gene ID 59109). Neurotrophins mediate a wide range of neuronal functions, including survival, differentiation, growth, and death, via two classes of transmembrane receptors, the Trk receptor tyrosine kinase and the p75 neurotrophin receptor. While nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and NT-4 all bind the p75 receptor, TrkA is selective for NGF, TrkB is selective for BDNF and NT-4, and TrkC is selective for NT-3. TrkA and TrkB receptors can also be activated by ligands of the G-protein-coupled receptor (GPCRs), including the nucleoside adenosine or adenosine agonists, such as CGS 21680 and the pituitary adenylate cyclase-activating polypeptide (PACAP). In addition to transactivation via GPCRs, Glucocorticoids (GCs) are also reported to promote TrkA/B/C activation due to signaling cross-talk between Trk and GC receptor (GR).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect phospho-TrkA (Tyr794) using this Anti-phospho-TrkA (Tyr794) Antibody validated for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected NGF-stimulated Tyr794 phosphorylation of exogenously expressed human TrkA in PC12 cells (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University).
      Immunocytochemistry Analysis: A representative lot detected PACAP-induced TrkA Tyr794 phosphorylation in cultured rat basal forebrain neurons (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University).
      Western Blotting Analysis: A representative lot detected NGF-stimulated Tyr794 phosphorylation of exogenously expressed human TrkA in PC12 cells (Rajagopal, R., et al. (2004). 24(30):6650-6658).
      Western Blotting Analysis: A representative lot detected NGF-stimulated TrkA Tyr794 phosphorylation in cultured rat embryonic E18 septal neurons (Jeanneteau, F., et al. (2008). Proc Natl Acad Sci USA. 105(12):4862-4867).
      Western Blotting Analysis: A representative lot detected NGF-dependent TrkA Tyr794 phosphorylation in cultured DRG neurons from E16.5 rat embryos (Arevalo, J.C., et al. (2006). Neuron. 50(4):549-559).
      Immunocytochemistry Analysis: A representative lot detected PACAP38-induced TrkA Tyr794 phosphorylation in cultured rat basal forebrain neurons, as well as CGS 21680- and NGF-induced Tyr794 phosphorylation of exogenously expfressed human TrkA in serum-starved PC12 cultures (Rajagopal, R., et al. (2004). 24(30):6650-6658).
      Immunocytochemistry Analysis: A representative lot detected NGF-stimulated TrkA Tyr794 phosphorylation in cultured DRG neurons from E16.5 rat embryos (Arevalo, J.C., et al. (2006). Neuron. 50(4):549-559).
      Biological Information
      ImmunogenLinear peptide corresponding to rat TrkA sequence near the C-terminus with phosphorylated Tyr794.
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody selectively detects NGF-induced pTyr794 phosphorylation of TrkA in cultured primary embryonic rat septal neurons, but not BDNF-induced pTyr816 phosphorylation of TrkB in rat hippocampal neurons (PMID 18347336).
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteRat, Human. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ntrk1
      • Trk
      • Trka
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~140 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NGF treated human TrkA-transfected PC12 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected phospho-TrkA (Tyr794) in 10 µg of NGF treated human TrkA-transfected PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-TrkA (Tyr794) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-TrkA (Tyr794) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-TrkA (Tyr794) - 33275803327580
      Anti-phospho-TrkA (Tyr794) -Q2574770Q2574770

      References

      Reference overviewPub Med ID
      Transactivation of Trk neurotrophin receptors by G-protein-coupled receptor ligands occurs on intracellular membranes.
      Rajagopal, R; Chen, ZY; Lee, FS; Chao, MV
      The Journal of neuroscience : the official journal of the Society for Neuroscience  24  6650-8  2004

      Show Abstract
      15282267 15282267

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage. (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies