07-1643 Anti-phospho-Akt (Tyr450) Antibody

07-1643
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, B, Ch, Eq WB, Enzyme Assays Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-1643
      DescriptionAnti-phospho-Akt (Tyr450) Antibody
      Alternate Names
      • AKT1 kinase
      • Protein kinase B
      • RAC-alpha serine/threonine-protein kinase
      • rac protein kinase alpha
      • v-akt murine thymoma viral oncogene homolog 1
      Background InformationAkt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Lambda phosphatase treated and untreated NIH/3T3 with Anisomycin, RAT2 with SRC, and MCF7 with EGF
      PresentationAffinity purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-Akt (Tyr450) Antibody is an antibody against phospho-Akt (Tyr450) for use in WB, EA.
      Key Applications
      • Western Blotting
      • Enzyme Assays
      Application NotesWestern Blot (SNAP ID) Analysis: 1:1,000 dilution from a previous lot detected AKT phosphorylated at Tyr450 in NIH/3T3 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phosphorylated AKT at and around Tyr450.
      EpitopePhosphorylated Tyr450
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes phosphorylated Akt at Tyr450.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Bovine
      • Chicken
      • Horse
      Species Reactivity NoteReacts with human, mouse, and rat. Predicted to react with cow, chicken, and horse based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      • AKT
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

      SIZE: 480 amino acids; 55686 Da

      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).

      TISSUE SPECIFICITY: In all human cell types so far analyzed.

      DOMAIN: Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).

      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular WeightApprox. 56 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in NIH/3T3, RAT2, and MCF7 cell lysates.

      Western Blot Analysis: 1:250-1,000 dilution of this antibody detected AKT phosphorylated at Tyr450 in NIH/3T3, RAT2, and MCF7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-Akt (Tyr450) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Akt (Tyr450) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-Akt (Tyr450) - 24546952454695
      Anti-phospho-Akt (Tyr450) - 21671762167176
      Anti-phospho-Akt (Tyr450) - 22071972207197
      Anti-phospho-Akt (Tyr450) - 25331382533138
      Anti-phospho-Akt (Tyr450) - NRG1660339NRG1660339
      Anti-phospho-Akt (Tyr450) -26258742625874
      Anti-phospho-Akt (Tyr450) -27974042797404
      Anti-phospho-Akt (Tyr450) Polyclonal Antibody3105310

      References

      Reference overviewPub Med ID
      Cyclic GMP signaling in rat urinary bladder, prostate, and epididymis: tissue-specific changes with aging and in response to Leydig cell depletion.
      Müller, D; Mukhopadhyay, AK; Davidoff, MS; Middendorff, R
      Reproduction (Cambridge, England)  142  333-43  2011

      Show Abstract
      21511885 21511885

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition (MilliporeSigma)

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      Life Science Research > Antibodies and Assays > Primary Antibodies