MABC32 Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2

MABC32
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M WB, FC, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC32
      DescriptionAnti-p62 (Sequestosome-1) Antibody, clone 11C9.2
      Alternate Names
      • sequestosome 1
      • EBI3-associated protein of 60 kDa
      • Paget disease of bone 3
      • phosphotyrosine independent ligand for the Lck SH2 domain p62
      • oxidative stress induced like
      • EBI3-associated protein p60
      • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
      • Ubiquitin-binding protein p62
      • sequestosome-1
      Background Informationp62 is also known as sequestosome-1, and is a 62 kDa ras-GAP associated protein. p62 is thought to bind to src-family tyrosine kinases and may also provide a linker action for activated src-family kinases with their downstream effectors. p62 has an RNA-binding region and may have RNA-binding ability. p62 is associated with activation of transcription factor NF-κB, involved in cell differentiation, immune response and potassium channel regulation. p62 has recently been linked to extrinsic apoptosis and may be a key player in tumorigenesis.
      References
      Product Information
      FormatPurified
      Control
      • A431 cell lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      Applications
      ApplicationDetect p62 (Sequestosome-1) using this Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 validated for use in WB, FC & IC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 0.001 µg/mL from a previous lot detected p62 (Sequestosome-1) in 10 µg of PC12 cell lysate.

      Flow Cytometry Analysis: 1.0 µg from a previous lot detected p62 (Sequestosome-1) in the staining of fixed and permeabilized HeLa cells.

      Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p62 (Sequestosome-1) in NIH/3T3, A431, and HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human p62 (Sequestosome-1).
      Clone11C9.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq].
      Gene Symbol
      • PDB3
      • p62B
      • p62
      • A170
      • ZIP3
      • SQSTM1
      • p60
      • OSIL
      • ORCA
      • EBIAP
      • P62
      Purification MethodIon Exchange
      UniProt Number
      UniProt SummaryFUNCTION: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

      SUBUNIT STRUCTURE: Homooligomer or heterooligomer; may form homotypic arrays. Interacts directly with PRKCI and PRKCZ Probable. Forms ternary complexes with PRKCZ and KCNAB2 or PRKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6 (By similarity). Interacts with EBI3, LCK, RASA1, PRKCZ, PRKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MAP2K5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63-polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MAP2K5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. Interacts with TRAF6 and CYLD. Identified in a complex with TRAF6 and CYLD (By similarity).

      SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Nucleus. Note: Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.

      TISSUE SPECIFICTY: Ubiquitously expressed.

      INDUCTION: By proteasomal inhibitor PSI and prostaglandin J2 (PGJ2) (at protein level). By phorbol 12-myristate 13-acetate (PMA).

      DOMAIN: The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.

      The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.

      The ZZ-type zinc finger mediates the interaction with RIPK1.

      PTM: Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.

      INVOLVEMENT IN DISEASE: Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.

      SEQUENCE SIMILARITIES: Contains 1 OPR domain.

      Contains 1 UBA domain.

      Contains 1 ZZ-type zinc finger.
      Molecular Weight~47-60 kDa observed.
      The calculated molecular weight of this protein is 47 kDa and also has an isoform at 38 kDa. Due to modifications, this protein may be observed up to ~60 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 0.001 µg/mL of this antibody detected p62 (Sequestosome-1) in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 Certificates of Analysis

      TitleLot Number
      Anti-p62 (Sequestosome-1), clone 11C9.2 - 21278402127840
      Anti-p62 (Sequestosome-1), -25275892527589
      Anti-p62 (Sequestosome-1), -26481812648181
      Anti-p62 (Sequestosome-1), -26913692691369
      Anti-p62 (Sequestosome-1), -27235522723552
      Anti-p62 (Sequestosome-1), -27463982746398
      Anti-p62 (Sequestosome-1), clone 11C9.22475925
      Anti-p62 (Sequestosome-1), clone 11C9.2 - 19530031953003
      Anti-p62 (Sequestosome-1), clone 11C9.2 - 19751061975106
      Anti-p62 (Sequestosome-1), clone 11C9.2 - 19918631991863

      References

      Reference overviewPub Med ID
      Suppression of experimental arthritis through AMP-activated protein kinase activation and autophagy modulation.
      Yan, H; Zhou, HF; Hu, Y; Pham, CT
      Journal of rheumatic diseases and treatment  1  5  2015

      Show Abstract
      26120598 26120598
      Fumagillin prodrug nanotherapy suppresses macrophage inflammatory response via endothelial nitric oxide.
      Zhou, HF; Yan, H; Hu, Y; Springer, LE; Yang, X; Wickline, SA; Pan, D; Lanza, GM; Pham, CT
      ACS nano  8  7305-17  2014

      Show Abstract
      24941020 24941020
      Lysosomal-mediated waste clearance in retinal pigment epithelial cells is regulated by CRYBA1/βA3/A1-crystallin via V-ATPase-MTORC1 signaling.
      Valapala, M; Wilson, C; Hose, S; Bhutto, IA; Grebe, R; Dong, A; Greenbaum, S; Gu, L; Sengupta, S; Cano, M; Hackett, S; Xu, G; Lutty, GA; Dong, L; Sergeev, Y; Handa, JT; Campochiaro, P; Wawrousek, E; Zigler, JS; Sinha, D
      Autophagy  10  480-96  2014

      Show Abstract
      24468901 24468901
      Human stefin B role in cell's response to misfolded proteins and autophagy.
      Polajnar, M; Zavašnik-Bergant, T; Škerget, K; Vizovišek, M; Vidmar, R; Fonović, M; Kopitar-Jerala, N; Petrovič, U; Navarro, S; Ventura, S; Žerovnik, E
      PloS one  9  e102500  2014

      Show Abstract
      25047918 25047918
      AMPK involvement in endoplasmic reticulum stress and autophagy modulation after fatty liver graft preservation: a role for melatonin and trimetazidine cocktail.
      Zaouali, Mohamed Amine, et al.
      J. Pineal Res., (2013)  2013

      Show Abstract
      23551302 23551302
      Tsc1 (hamartin) confers neuroprotection against ischemia by inducing autophagy.
      Papadakis, M; Hadley, G; Xilouri, M; Hoyte, LC; Nagel, S; McMenamin, MM; Tsaknakis, G; Watt, SM; Drakesmith, CW; Chen, R; Wood, MJ; Zhao, Z; Kessler, B; Vekrellis, K; Buchan, AM
      Nature medicine  19  351-7  2013

      Show Abstract
      23435171 23435171

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