PC322L Anti-VEGFR1 Goat pAb

PC322L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Gt Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC322L-100UG
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes VEGF receptor 1.
          Catalogue NumberPC322L
          Brand Family Calbiochem®
          SynonymsAnti-Vascular Endothelial Growth Factor Receptor, Anti-VEGF-R1, Anti-Flt-1, Anti-EMRK2
          References
          ReferencesSoker, S., et al. 1998. Cell 92, 735.
          Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
          Kendall, R.L. and Thomas, K.A. 1993. PNAS. 90, 10705.
          Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
          Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
          Brown, L., et al. 1992. Kidney International 42, 1457.
          Product Information
          FormLyophilized
          FormulationLyophilized from a sterile solution in PBS, 5% trehalose.
          Positive controlEndothelial cells
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Competition Studies
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesCompetition Studies (see comments)
          ELISA (0.5-1 µg/ml)
          Immunoblotting (0.1-0.2 µg/ml)
          Paraffin Sections (5-15 µg/ml)
          Application CommentsThis antibody has been selected for its ability to recognize human recombinant VEGFR-1 in immunoblotting and ELISA. The detection limit for human recombinant VEGFR-1 by immunoblotting is approximately 50 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant Flt-1 by ELISA is approximately 0.16 ng/well. At approximately 10 µg/ml this antibody will completely block the binding of rhPlGF (2 ng/ml) to immobilized rhVEGFR1/Fc (1 µg/ml; 100 µl/well). This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, human VEGFR1 (extracellular domain)/Fc chimera
          ImmunogenHuman
          HostGoat
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody in 1 ml sterile PBS. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-VEGFR1 Goat pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-VEGFR1 Goat pAb Certificates of Analysis

          TitleLot Number
          PC322L

          References

          Reference overview
          Soker, S., et al. 1998. Cell 92, 735.
          Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
          Kendall, R.L. and Thomas, K.A. 1993. PNAS. 90, 10705.
          Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
          Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
          Brown, L., et al. 1992. Kidney International 42, 1457.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-July-2007 RFH
          SynonymsAnti-Vascular Endothelial Growth Factor Receptor, Anti-VEGF-R1, Anti-Flt-1, Anti-EMRK2
          ApplicationCompetition Studies (see comments)
          ELISA (0.5-1 µg/ml)
          Immunoblotting (0.1-0.2 µg/ml)
          Paraffin Sections (5-15 µg/ml)
          DescriptionImmunoaffinity purified goat polyclonal antibody. Recognizes the VEGF receptor 1 protein.
          BackgroundVascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a secreted heparin binding cytokine expressed in tumor and animal cells. VEGF has been implicated in the control of angiogenesis due to its selective mitogenic stimulation of vascular endothelial cells and enhancement of vascular permeability. VEGF is a ~34-50 kDa dimer of two identical disulfide-linked subunits existing in four molecular species; 121, 165, 189, and 206 amino acid residues long, arising from alternative splicing of a single gene. VEGF binds to two receptor tyrosine kinases, Flt-1 (VEGFR1) and KDR/Flk-1 (VEGFR2) found almost exclusively on human endothelial cells. Flt-1 binds VEGF and a new VEGF-related ligand, placenta growth factor (PIGF) but KDR/Flk-1 binds only VEGF. The cDNA encoding the soluble truncated form of Flt-1 cloned from a human endothelial cell library encodes the 6 N-terminal IgG-like extracellular ligand-binding domain but does not encode the transmembrane spanning region and intracellular tyrosine kinase domains. The recombinant soluble receptor binds avidly to VEGF and is a potent VEGF antagonist. Recently, a third VEGF receptor was identified and cloned from tumor cells. This VEGF receptor is isoform-specific, binding VEGF165 but not VEGF121. Studies indicate that this VEGF receptor (VEGF165R) is identical to human neuropilin-1, a receptor for the collapsin/semaphorin family that mediates neuronal cell guidance.
          HostGoat
          Immunogen speciesHuman
          Immunogenrecombinant, human VEGFR1 (extracellular domain)/Fc chimera
          IsotypeIgG
          Specieshuman
          Positive controlEndothelial cells
          FormLyophilized
          FormulationLyophilized from a sterile solution in PBS, 5% trehalose.
          PreservativeNone
          CommentsThis antibody has been selected for its ability to recognize human recombinant VEGFR-1 in immunoblotting and ELISA. The detection limit for human recombinant VEGFR-1 by immunoblotting is approximately 50 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant Flt-1 by ELISA is approximately 0.16 ng/well. At approximately 10 µg/ml this antibody will completely block the binding of rhPlGF (2 ng/ml) to immobilized rhVEGFR1/Fc (1 µg/ml; 100 µl/well). This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody in 1 ml sterile PBS. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesSoker, S., et al. 1998. Cell 92, 735.
          Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
          Kendall, R.L. and Thomas, K.A. 1993. PNAS. 90, 10705.
          Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
          Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
          Brown, L., et al. 1992. Kidney International 42, 1457.