CB1052 Anti-TUBB2A Mouse mAb (2B2)

CB1052
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      CB1052-100UG
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      Discontinued
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          100 μg
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          Description
          OverviewRecognizes the ~48-50 kDa TUBB2A protein in PC-12, Raw 264.7, Jurkat, and NIH3T3 cells and human spleen tissue.
          Catalogue NumberCB1052
          Brand Family Calbiochem®
          SynonymsAnti-Tubulin β2A
          Application Data
          Detection of rat TUBB2A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse TUBB2A by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human TUBB2A by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human TUBB2A by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1.5 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlPC-12 cells, Raw 264.7 cells, Jurkat cells, NIH3T3 cells, Human spleen tissue
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesImmunoblotting (1-5 µg/ml)
          Paraffin Sections (1.5 µg/ml)
          Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant, human TUBB2A (aa 1-446), expressed as a GST fusion protein
          Clone2B2
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-TUBB2A Mouse mAb (2B2) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-November-2010 RFH
          SynonymsAnti-Tubulin β2A
          ApplicationImmunoblotting (1-5 µg/ml)
          Paraffin Sections (1.5 µg/ml)
          Application Data
          Detection of rat TUBB2A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse TUBB2A by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human TUBB2A by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human TUBB2A by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1.5 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~48-50 kDa TUBB2A protein.
          BackgroundTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the α-chain.
          HostMouse
          ImmunogenFull-length, recombinant, human TUBB2A (aa 1-446), expressed as a GST fusion protein
          Clone2B2
          IsotypeIgG2a
          Specieshuman, mouse, rat
          Positive controlPC-12 cells, Raw 264.7 cells, Jurkat cells, NIH3T3 cells, Human spleen tissue
          Negative control293T cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review