GF16 Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2)

GF16
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      GF16-200UG
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      Discontinued
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      Available
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          Contact Customer Service

          Plastic ampoule 200 μg
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          Description
          OverviewRecognizes TGF-β2 and TGF-β3 in umbilical cord cells. Does not cross-react with TGF-β1.
          Catalogue NumberGF16
          Brand Family Calbiochem®
          SynonymsAnti-Transforming Growth Factor-β3
          References
          ReferencesBurton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
          Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
          ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
          ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
          de Martin, R., et al. 1987 EMBO J. 6, 3677.
          Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
          Derynck, R., et al. 1985 Nature 316, 701.
          Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
          Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
          Positive controlRecombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesFrozen Sections (1-5 µg/ml)
          Immunoblotting (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
          Application CommentsDoes not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton® X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, human TGF-β3
          ImmunogenHuman
          Epitopewithin the N-terminal region
          Clone236-5.2
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) Certificates of Analysis

          TitleLot Number
          GF16

          References

          Reference overview
          Burton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
          Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
          ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
          ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
          de Martin, R., et al. 1987 EMBO J. 6, 3677.
          Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
          Derynck, R., et al. 1985 Nature 316, 701.
          Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
          Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.

          Brochure

          Title
          TGF-b Signaling: Dual Role in Tumor Suppression and Oncogenesis (EMD)
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision23-August-2007 RFH
          SynonymsAnti-Transforming Growth Factor-β3
          ApplicationFrozen Sections (1-5 µg/ml)
          Immunoblotting (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
          DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x63 Ag 8.653 myeloma cells. Recognizes TGF-β2 and TGF-β3.
          BackgroundTransforming growth factorβs are a family of proteins originally characterized by their ability to act synergistically with EGF or TGFα. TGFβs have been found to produce multiple biological effects including mitogenesis, growth inhibition, chemotaxis and induction or inhibition of differentiation. In their mature form TGFβs are acid- and heat-stable disulfide-linked homodimeric proteins (Mr = ~25 kDa), with each homodimer consisting of 112 amino acid residues. Two homodimeric forms of TGFβ have been identified (TGFβ1 and TGFβ2) which show significant amino acid sequence homology. A new member of the family, TGFβ3 has recently been cloned and characterized. There is greater amino acid homology between TGFβ3 and TGFβ2 as compared with TGFβ1. All TGFβs are released by cells in a biologically latent form. Most cells have high affinity receptors for TGFβ1, β2, and β3.
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant, human TGF-β3
          Epitopewithin the N-terminal region
          Clone236-5.2
          IsotypeIgG₁
          Specieshuman, mouse, rat
          Positive controlRecombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsDoes not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton® X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBurton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
          Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
          ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
          ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
          de Martin, R., et al. 1987 EMBO J. 6, 3677.
          Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
          Derynck, R., et al. 1985 Nature 316, 701.
          Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
          Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.