ST1709 Anti-SKP1A Mouse mAb (1H8)

ST1709
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1709-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~18 kDa SKP1A protein in HeLa cells.
          Catalogue NumberST1709
          Brand Family Calbiochem®
          SynonymsAnti-S-Phase Kinase-Associated Protein 1
          Application Data
          Detection of human SKP1A by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human SKP1A by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (10 µg/ml). Detection: fluorescence.
          References
          Product Information
          Formliquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlHeLa cells
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesImmunoblotting (1-5 µg/ml
          Immunocytochemistry (10 µg/ml)
          Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids of 53-161 human SKP1A, expressed as a GST fusion protein
          Clone1H8
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-SKP1A Mouse mAb (1H8) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-November-2010 RFH
          SynonymsAnti-S-Phase Kinase-Associated Protein 1
          ApplicationImmunoblotting (1-5 µg/ml
          Immunocytochemistry (10 µg/ml)
          Application Data
          Detection of human SKP1A by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human SKP1A by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (10 µg/ml). Detection: fluorescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~18 kDa SKP1A protein.
          BackgroundThis gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids of 53-161 human SKP1A, expressed as a GST fusion protein
          Clone1H8
          IsotypeIgG2a
          Specieshuman
          Positive controlHeLa cells
          Negative control293T cells
          Formliquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review