07-1464 Anti-Rac1 Antibody

07-1464
100 µg  
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      Overview

      Replacement Information
      Replacement Information07-1464 is a recommended replacement for AB4202

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-1464
      ReplacesAB4202
      DescriptionAnti-Rac1 Antibody
      Alternate Names
      • Ras-related C3 Botulinum Toxin Substrate 1
      • p21-Rac1
      • Ras-like Protein TC25
      Background InformationRac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.
      References
      Product Information
      FormatPurified
      Control
      • Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
        Immunohistochemistry: Rat brain sections
      PresentationPurified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative
      Applications
      ApplicationAnti-Rac1 Antibody is an antibody against Rac1 for use in WB.
      Key Applications
      • Western Blotting
      Application NotesOptimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 130-141 of human Rac1 (KEKKLTPITYPQ-C).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Rac1, does not cross react with Cdc42. Rac1 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Rac regulates actin polymerization to form lamellipodia.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
      Gene Symbol
      • RAC1
      • ORFNames=MIG5
      • TC-25
      • p21-Rac1
      • MGC111543
      • PAK1
      • MIG5
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
      FUNCTION: Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
      ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
      SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with NISCH (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
      Molecular Weight21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on L6 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Rac1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Rac1 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Rac1 - 21105642110564
      Anti-Rac1 - 23904562390456
      Anti-Rac1 - 20129752012975
      Anti-Rac1 - 21471372147137
      Anti-Rac1 - 22943712294371
      Anti-Rac1 - 24888372488837
      Anti-Rac1 - 33077943307794
      Anti-Rac1 - JBC1787319JBC1787319
      Anti-Rac1 - NG1605306NG1605306
      Anti-Rac1 - NG1605308NG1605308

      References

      Reference overviewApplicationPub Med ID
      The matricellular protein CCN1 induces fibroblast senescence and restricts fibrosis in cutaneous wound healing.
      Jun, JI; Lau, LF
      Nature cell biology  12  676-85  2010

      Show Abstract
      Western Blotting20526329 20526329

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies