DR1015 Anti-RENT1 Goat pAb

DR1015
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Gt Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DR1015-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~140 kDa RENT1 protein in HeLa cells.
          Catalogue NumberDR1015
          Brand Family Calbiochem®
          SynonymsAnti-HUPF1, Anti-NORF1
          Application Data
          Detection of human RENT1 by immunoblotting. Samples: Whole cell lysate from untreated (-) and irradiated (+) lymphophoid cell lines (LCL1 and LCL2; EBV-immortalized B-cell lines from a normal individuals). Primary antibody: Anti-RENT1 Goat pAb (Cat. No. DR1015) (1:1000). Detection: chemiluminescence.
          References
          ReferencesMendell, J.T., et al. 2002. Science 298, 419.
          Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.
          Product Information
          FormLiquid
          FormulationIn Tris/citrate phosphate buffer, pH 7.0-8.0.
          Positive controlHeLa cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000-1:2500)
          Application CommentsMay detect an additional band at ~80 kDa. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human RENT1
          ImmunogenHuman
          HostGoat
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Highly Toxic
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-RENT1 Goat pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-RENT1 Goat pAb Certificates of Analysis

          TitleLot Number
          DR1015

          References

          Reference overview
          Mendell, J.T., et al. 2002. Science 298, 419.
          Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-August-2007 RFH
          SynonymsAnti-HUPF1, Anti-NORF1
          ApplicationImmunoblotting (1:1000-1:2500)
          Application Data
          Detection of human RENT1 by immunoblotting. Samples: Whole cell lysate from untreated (-) and irradiated (+) lymphophoid cell lines (LCL1 and LCL2; EBV-immortalized B-cell lines from a normal individuals). Primary antibody: Anti-RENT1 Goat pAb (Cat. No. DR1015) (1:1000). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified goat polyclonal antibody. Recognizes the ~140 kDa RENT1 protein.
          BackgroundEukaryotes have the ability to recognize and degrade transcripts that harbor premature signals for translational termination. Nonsense and frameshift mutations that result in these premature termination codons play a role in many genetic diseases and some cancers. Nonsense-mediated nRNA decay (NMD) is mediated by several factors including rent1/hUpf1, rent2/hUpf2, and hUpf3. When assembled, these proteins form the surveillence complex. Disruption of the mouse Rent1 gene has been reported to completely disrupt NMD and result in embryos that re-absorb shortly after implantation.
          HostGoat
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human RENT1
          IsotypeIgG
          Specieshuman
          Positive controlHeLa cells
          FormLiquid
          FormulationIn Tris/citrate phosphate buffer, pH 7.0-8.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsMay detect an additional band at ~80 kDa. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
          Toxicity Highly Toxic
          ReferencesMendell, J.T., et al. 2002. Science 298, 419.
          Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.