MABN768 Anti-PrP, clone DRM2-118 Antibody

MABN768
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, B, Sh, Ht, Porcine, H WB, IHC, ELISA M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABN768
      DescriptionAnti-PrP, clone DRM2-118 Antibody
      Alternate Names
      • Major prion protein
      • PrP
      • PrP27-30
      • PrP33-35C
      • CD230
      Background InformationThe protein named PrP is the cellular gene for the prion protein. The normal form appears to play a role in neuronal development and synaptic plasticity, iron transport and myelin sheath maintenance. PrP has both membrane associated and cytoplasmic/nuclear isoforms. The primary isoform is localized to the cellular membrane in lipid anchor clusters, as well as in the Golgi. Isoform 2 is cytoplasmic that becomes localized to the nucleus. Prp is normally a monomeric protein but a disease associated mutated form will form protease resistant oligomers often with another protein beta amyloid. The mutated form is found in high quantity in the brain of humans and animals infected with the degenerative neurological diseases such as Kuru, Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler syndrome (GSS), Scrapie, and Bovine Spongiform Encephalopathy (BSE) and Transmissible Mink Encephalopathy.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-PrP, clone DRM2-118 | MABN768 is an antibody against PrP for use in Western Blotting, Immunohistochemistry, ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected PrP in 10 µg of rat brain tissue lysate.
      ELISA Analysis: A representative lot detected PrP in recombinant PrP proteins from multiple species by ELISA using DRM1-31, DRM1-60, and DRM2-118 (Stanker, L.H., et al. (2012). 31(5):314-24).
      Immunohistochemistry Analysis: A representative lot detected PrP in hamster brain sections tissue with DRM1-31, DRM1-60, and DRM2-118 (Stanker, L.H., et al. (2012). 31(5):314-24).
      Western Blotting Analysis: A representative lot detected PrP in normal brain extract from multiple spcecies using DRM2-118. DRM1-60, and DRM1-31 lysate (Stanker, L.H., et al. (2012). 31(5):314-24).
      Biological Information
      ImmunogenRecombinant protein corresponding to syrian hamster PrP.
      CloneDRM2-118
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityDRM1-60 showed strong binding to only one peptide (#29: NQVYYRPVDQYS) suggesting a linear epitope corresponding to SHaPrP(159–170), located in b2-a2 loop. This epitope assignment is consistent with the binding of DRM1-60 to PrPC to all species tested, except human and horse, which both harbor substitutions in this region. While antibodies with epitopes similar to DRM2-118 have been described,(36) DRM1-60 and DRM1-31 have unique epitopes not previously reported. DRM1-60 appears to bind a linear epitope localized to the b2-a2 loop, while DRM1-31 has a conformational epitope located on the exposed surface formed by the close proximity of the b2-a2- helix loop, the N-terminus of the a2 helix, and the C-terminus of the a3 helix.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Rat
      • Bovine
      • Sheep
      • Hamster
      • Porcine
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRNP
      • PRP
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~36 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PrP in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-PrP, clone DRM2-118 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PrP, clone DRM2-118 Antibody Certificates of Analysis

      TitleLot Number
      Anti-PrP, clone DRM2-118 -Q2499610Q2499610

      References

      Reference overviewPub Med ID
      Hybridoma (2005)
      Stanker, LH; Scotcher, MC; Lin, A; McGarvey, J; Prusiner, SB; Hnasko, R
      Hybridoma (2005)  31  314-24  2012

      Show Abstract
      23098297 23098297

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies