OP38 Anti-Pan-Ras (Ab-1) Mouse mAb (DWP)

OP38
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP38-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~21 kDa H-, K-, and N-Ras proteins.
          Catalogue NumberOP38
          Brand Family Calbiochem®
          References
          ReferencesHamer, P.J., et al. 1991 Oncogene 6, 1609.
          Ng, S., et al. 1991. Hybridoma 10, 467.
          Hamer, P.J. et al. 1990. Hybridoma 9, 573.
          Pullano, T.G., et al. 1989. Oncogene 4, 1003.
          Bos, J.L., 1988. Mutation Res. 195, 255.
          Furth, M.E., et al. 1987. Oncogene 1, 47.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Positive controlK-Ras Val¹² Protein (Cat. No. WA04)
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesOriginal Clone
          Carney, W.P. et al. 1986. Proc. Natl. Acad. Sci., USA 83, 7485.
          Specificity
          Wang, Z., et al. 1997. J. Peptide Res. 50, 483.
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Application CommentsRecognizes H-, K-, and N-Ras proteins. Per Wang, Z., et al. 1997, this clone may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 5-16 of Ras (H, K, or N) with Val at position 12
          CloneDWP
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Pan-Ras (Ab-1) Mouse mAb (DWP) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Pan-Ras (Ab-1) Mouse mAb (DWP) Certificates of Analysis

          TitleLot Number
          OP38

          References

          Reference overview
          Hamer, P.J., et al. 1991 Oncogene 6, 1609.
          Ng, S., et al. 1991. Hybridoma 10, 467.
          Hamer, P.J. et al. 1990. Hybridoma 9, 573.
          Pullano, T.G., et al. 1989. Oncogene 4, 1003.
          Bos, J.L., 1988. Mutation Res. 195, 255.
          Furth, M.E., et al. 1987. Oncogene 1, 47.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-September-2010 JSW
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c x C57BL/6 mice with the specified immunogen and fusing splenocytes with Sp2/0 myeloma cells (see application references). Recognizes the ~21 kDa Ras protein.
          BackgroundThe human ras gene family consists of three identified members H, K and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons weight. Human H-and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and its relevance to human disease diagnosis and prognosis remains to be determined.
          HostMouse
          Immunogena synthetic peptide corresponding to amino acids 5-16 of Ras (H, K, or N) with Val at position 12
          CloneDWP
          IsotypeIgG2b
          Specieshuman, mouse, rat
          Positive controlK-Ras Val¹² Protein (Cat. No. WA04)
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsRecognizes H-, K-, and N-Ras proteins. Per Wang, Z., et al. 1997, this clone may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesHamer, P.J., et al. 1991 Oncogene 6, 1609.
          Ng, S., et al. 1991. Hybridoma 10, 467.
          Hamer, P.J. et al. 1990. Hybridoma 9, 573.
          Pullano, T.G., et al. 1989. Oncogene 4, 1003.
          Bos, J.L., 1988. Mutation Res. 195, 255.
          Furth, M.E., et al. 1987. Oncogene 1, 47.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.
          Application referencesOriginal Clone
          Carney, W.P. et al. 1986. Proc. Natl. Acad. Sci., USA 83, 7485.
          Specificity
          Wang, Z., et al. 1997. J. Peptide Res. 50, 483.