PH03L Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D)

PH03L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PH03L-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
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          Will advise
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          Contact Customer Service

          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the T-cell PTPase protein.
          Catalogue NumberPH03L
          Brand Family Calbiochem®
          SynonymsAnti-Protein Tyrosine Phosphatase, T cell
          References
          ReferencesBrown-Shimer, S., et al. 1992. Can. Res. 52, 478.
          Fischer, E.H., et al. 1991 Science 253, 401.
          Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
          Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
          Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
          Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
          Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
          Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.
          Product Information
          FormLyophilized
          FormulationLyophilized from 20 mM sodium bicarbonate, 100 µg BSA.
          Positive controlCCRF-CEM cells
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesImmunocytochemistry (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Application CommentsThis antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, human T cell PTPase
          ImmunogenHuman
          CloneCF4-1D
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) Certificates of Analysis

          TitleLot Number
          PH03L

          References

          Reference overview
          Brown-Shimer, S., et al. 1992. Can. Res. 52, 478.
          Fischer, E.H., et al. 1991 Science 253, 401.
          Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
          Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
          Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
          Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
          Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
          Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.

          Brochure

          Title
          Protein Phosphatases Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-October-2007 RFH
          SynonymsAnti-Protein Tyrosine Phosphatase, T cell
          ApplicationImmunocytochemistry (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the PTPase protein.
          BackgroundProtein tyrosine phosphatases (PTPases) make up an expanding family of transmembrane and intracellular proteins, which function to remove phosphate groups from protein phosphoryl- tyrosine residues. The prototype phosphatase, PTPase 1B, was initially identified as an active intracellular phosphatase from human placenta made up of 321 amino acids and subsequently shown to derive from a larger precursor protein containing 435 amino acids. Using probes to the conserved PTPase domain a cDNA clone was isolated from a human peripheral T cell library encoding a protein with 65% identity to the placental PTPase 1B. The T cell protein differs in amino acid composition but is highly similar with respect to hydrophobicity with the 1B protein and is now known to be present in other cell types. While the function of the T cell PTPase protein is still unknown, recent experiments demonstrated that a truncated form overexpressed in BHK cells resulted in a greatly reduced growth rate of the cells. PTPases are involved in regulating processes where protein kinases and protein tyrosine phosphorylation are important. One intriguing hypothesis is that PTPases have the potential to function as anti-oncogenes.
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant, human T cell PTPase
          CloneCF4-1D
          IsotypeIgG2a
          Specieshuman, not mouse
          Positive controlCCRF-CEM cells
          FormLyophilized
          FormulationLyophilized from 20 mM sodium bicarbonate, 100 µg BSA.
          PreservativeNone
          CommentsThis antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesBrown-Shimer, S., et al. 1992. Can. Res. 52, 478.
          Fischer, E.H., et al. 1991 Science 253, 401.
          Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
          Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
          Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
          Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
          Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
          Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.