ABS234 Anti-PI3 Kinase Antibody, p85

200 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R WB, IP Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABS234
      DescriptionAnti-PI3 Kinase Antibody, p85
      Alternate Names
      • Phosphatidylinositol 3-kinase regulatory subunit alpha
      • PI3-kinase regulatory subunit alpha
      • PI3K regulatory subunit alpha
      • PtdIns-3-kinase regulatory subunit alpha
      • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
      • PI3-kinase subunit p85-alpha
      • PtdIns-3-kinase regulatory subunit p85-alpha
      Background InformationPhosphatidylinositol 3-Kinase (PI3 Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β,γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibited by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
      Product Information
      FormatAffinity Purified
      • Jurkat cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect PI3 Kinase, p85 using this rabbit polyclonal antibody, Anti-PI3 Kinase Antibody, p85 validated for use in western blotting & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated PI3 Kinase, p85 in 0.5 mg of Jurkat cell lysate.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to rat PI3 Kinase, p85.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with Human, and Rat. Predicted to react with Mouse, Bovine, and Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pik3r1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~85 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:600 dilution of this antibody detected PI3 Kinase, p85 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information


      Anti-PI3 Kinase Antibody, p85 SDS


      Safety Data Sheet (SDS) 

      Anti-PI3 Kinase Antibody, p85 Certificates of Analysis

      TitleLot Number
      Anti-PI3 Kinase p85 - 32016943201694
      Anti-PI3 Kinase p85 Polyclonal Antibody3153268
      Anti-PI3 Kinase, p85 - 25271962527196
      Anti-PI3 Kinase, p85 - Q2187161Q2187161
      Anti-PI3 Kinase, p85 -25770522577052
      Anti-PI3 Kinase, p85 -26088532608853
      Anti-PI3 Kinase, p85 -26253662625366
      Anti-PI3 Kinase, p85 -26877982687798
      Anti-PI3 Kinase, p85 -27300232730023
      Anti-PI3 Kinase, p85 -27598172759817

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      Life Science Research > Antibodies and Assays > Primary Antibodies