PC474 Anti-PCNA (247-261) (Ab-5) Rabbit pAb

PC474
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC474-50UL
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 50 ul
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~37 kDa PCNA protein in MCF-7 cells.
          Catalogue NumberPC474
          Brand Family Calbiochem®
          SynonymsAnti-Proliferating Cell Nuclear Antigen
          Application Data
          Detection of human PCNA by immunoprecipitation. Sample: Whole cell lysate from MCF7 cells. Antibodyfor IP: Anti-PCNA (Ab-1) Mouse mAb (PC10) (Cat. No. NA03) (1 µg/ml). Anti-PCNA (247-261) (Ab-5) Rabbit pAb (Cat. No. PC474) (1:1000). Detection: chemiluminescence. A nonspecific band appears at ~45 kDa in the sample lysate and in all the controls.
          References
          ReferencesMiyachi, K., et al. 1990. J. Immunol. 121, 2228.
          Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
          Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
          Bravo, R., et al. 1987. Nature (London) 326, 515.
          Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
          Product Information
          FormLiquid
          FormulationUndiluted serum.
          Positive controlMCF7 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Application NotesImmunoprecipitation (1:250-1:1000)
          Application CommentsWill immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 247-261 of human PCNA
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-PCNA (247-261) (Ab-5) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-PCNA (247-261) (Ab-5) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC474

          References

          Reference overview
          Miyachi, K., et al. 1990. J. Immunol. 121, 2228.
          Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
          Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
          Bravo, R., et al. 1987. Nature (London) 326, 515.
          Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-March-2008 JSW
          SynonymsAnti-Proliferating Cell Nuclear Antigen
          ApplicationImmunoprecipitation (1:250-1:1000)
          Application Data
          Detection of human PCNA by immunoprecipitation. Sample: Whole cell lysate from MCF7 cells. Antibodyfor IP: Anti-PCNA (Ab-1) Mouse mAb (PC10) (Cat. No. NA03) (1 µg/ml). Anti-PCNA (247-261) (Ab-5) Rabbit pAb (Cat. No. PC474) (1:1000). Detection: chemiluminescence. A nonspecific band appears at ~45 kDa in the sample lysate and in all the controls.
          DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~36-37 kDa PCNA protein.
          BackgroundThe proliferating cell nuclear antigen (PCNA) is a ~36-37 kDa protein also known as cyclin. The protein has been identified as a polymerase δ accessory protein and is detected in a cell cycle dependent manner. In early S phase PCNA has a very granular distribution and is absent from the nucleloli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cell fixed with aldehydes the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells. Patients suffering from SLE have been shown to have autoantibodies against PCNA.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids 247-261 of human PCNA
          IsotypeIgG
          Specieshuman
          Positive controlMCF7 cells
          FormLiquid
          FormulationUndiluted serum.
          Preservative≤0.1% sodium azide
          CommentsWill immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesMiyachi, K., et al. 1990. J. Immunol. 121, 2228.
          Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
          Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
          Bravo, R., et al. 1987. Nature (London) 326, 515.
          Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.