PC165L Anti-Opioid μ Receptor (384-398) Rabbit pAb

PC165L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC165L-100UL
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      Stocked 
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      Available
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~44-45 kDa opiod μ receptor in caudate putamen and the dorsal horn of the spinal cord.
          Catalogue NumberPC165L
          Brand Family Calbiochem®
          References
          ReferencesZaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol. 36, 379.
          Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.
          Kieffer, B.L. 1995. Cell. Mol. Neurobiol. 15, 615-635.
          Childers, S.R. 1991. Life Sci. 48, 1991.
          Product Information
          FormLyophilized
          FormulationUndiluted serum.
          Positive controlRat caudate putamen or spinal cord (dorsal horn)
          PreservativeNone
          Applications
          Application ReferencesImmunoblotting Wang, X., et al. 2004. Neurosci. 129, 751. Immunoblotting, Immunofluorescence, Frozen Sections Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.
          Key Applications Immunoprecipitation
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesFrozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP)
          Immunoblotting (1:2000-1:2500; see application references)
          Immunofluorescence (1:100-1:200)
          Immunoprecipitation (see comments)
          Application CommentsThe specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Opioid μ Receptor (384-398) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Opioid μ Receptor (384-398) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC165L

          References

          Reference overview
          Zaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol. 36, 379.
          Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.
          Kieffer, B.L. 1995. Cell. Mol. Neurobiol. 15, 615-635.
          Childers, S.R. 1991. Life Sci. 48, 1991.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2008 RFH
          ApplicationFrozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP)
          Immunoblotting (1:2000-1:2500; see application references)
          Immunofluorescence (1:100-1:200)
          Immunoprecipitation (see comments)
          DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~44-45 kDa opioid µ receptor protein.
          BackgroundOpioid peptides are endogenous neuromodulators, which have been identified from brain, and play a major role in the nociceptive pathway by interacting with several membrane receptors, defined as µ, δ, and κ. These peptides are derived from the cleavage of precursor proteins to produce the β-endorphin, enkephalin, and dynorphin peptides. Leu-enkephalin, Met-enkephalin, and β-endorphin all bind the µ and δ receptors with high affinity, while β-endorphin also exhibits low affinity κ binding. Dynorphin A and dynorphin B both bind the κ receptor with high affinity. In addition to these endogenous peptides, a number of exogenous nonpeptide molecules known as alkaloids or opiates also interact with the receptors to mediate a number of biological events which include the modulation of pain, analgesia, behavior, locomotor activity, and neuroendocrine physiology. Although the δ opioid receptor subtype was the first subtype identified, all three receptor classes are G protein-coupled receptors which have been shown to inhibit adenylate cyclase, decrease the conductance of voltage-gated Ca2+ channels, or activate K+ channel current.
          HostRabbit
          Immunogen speciesRat
          Immunogena synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor
          IsotypeIgG
          Speciesrat
          Positive controlRat caudate putamen or spinal cord (dorsal horn)
          FormLyophilized
          FormulationUndiluted serum.
          PreservativeNone
          CommentsThe specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C).
          Toxicity Highly Toxic
          ReferencesZaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol. 36, 379.
          Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.
          Kieffer, B.L. 1995. Cell. Mol. Neurobiol. 15, 615-635.
          Childers, S.R. 1991. Life Sci. 48, 1991.
          Application referencesImmunoblotting Wang, X., et al. 2004. Neurosci. 129, 751. Immunoblotting, Immunofluorescence, Frozen Sections Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.