481001 Anti-Neutrophil Elastase Rabbit pAb

481001
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      481001-1ML
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      Stocked 
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          Plastic ampoule 1 ml
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          Description
          OverviewRecognizes the neutrophil elastase protein.
          Catalogue Number481001
          Brand Family Calbiochem®
          Application Data
          Detection of human neutrophil elastase by immunoblotting. Samples: Low molecular weight standard (lane 1) and reduced elastase (lane 2) or non-reduced elastase (lane 3). Primary antibody: Anti-Neutrophil Elastase Rabbit pAb (Cat. No. 481001) (1:1000). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.5.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoelectrophoresis
          Application NotesELISA (≥ 1:1000)
          Immunoblotting (see comments)
          Immunoelectrophoresis (see comments)
          Immunoprecipitation (see comments)
          Application CommentsSingle arc by immunoelectrophoresis against neutrophil granule extract and human neutrophil elastase. This antibody has also been reported to work for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenhuman neutrophil elastase
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          481001

          Brochure

          Title
          Antibody Sourcebook!" Second Edition

          Citations

          Title
        • Auinash Kalsotra, et al. (2008) Inflammation resolved by retinoid X receptor-mediated inactivation of leukotriene signaling pathways. Federation of American Societies for Experimental Biology Journal 22, 538-547.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2007 RFH
          ApplicationELISA (≥ 1:1000)
          Immunoblotting (see comments)
          Immunoelectrophoresis (see comments)
          Immunoprecipitation (see comments)
          Application Data
          Detection of human neutrophil elastase by immunoblotting. Samples: Low molecular weight standard (lane 1) and reduced elastase (lane 2) or non-reduced elastase (lane 3). Primary antibody: Anti-Neutrophil Elastase Rabbit pAb (Cat. No. 481001) (1:1000). Detection: chemiluminescence.
          DescriptionAmmonium sulfate precipitated rabbit polyclonal antibody. Recognizes human neutrophil elastase.
          HostRabbit
          Immunogen speciesHuman
          Immunogenhuman neutrophil elastase
          IsotypeIgG
          Specieshuman
          FormLiquid
          FormulationIn PBS, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsSingle arc by immunoelectrophoresis against neutrophil granule extract and human neutrophil elastase. This antibody has also been reported to work for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          Citation
        • Auinash Kalsotra, et al. (2008) Inflammation resolved by retinoid X receptor-mediated inactivation of leukotriene signaling pathways. Federation of American Societies for Experimental Biology Journal 22, 538-547.