AB5922 Anti-Nestin Antibody

AB5922
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC, IHC, IH(P), WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB5922
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nestin Antibody
      Alternate Names
      • Nestin
      Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
      References
      Product Information
      FormatSerum
      Control
      • Positive controls are U251 or U373 human glioma cell lines. Detects a ~200-220 kDa protein, note slightly difference sizes for nestin are quoted in various paper and from various cell lines
      • in WI-38 (human lung line) reduced nestin migrates at ~220 kDa
      • in U251 (Human Glioma line) nestin migrates at ~260 kDa.
      PresentationRabbit polyclonal Serum in buffer containing Liquid.
      Applications
      ApplicationAnti-Nestin Antibody is an antibody against Nestin for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blot:
      1:1,000-1:5,000 dilution of a previous lot was used on cultured cells or human brain tissue. It is suggested that the membrane be blocked with PBS containing 5% nonfat milk and 0.05% Tween. The antibody may also be diluted in the same blocking buffer.

      Immunocytochemistry:
      1:200 dilution of a previous lot was used on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton.

      Immunohistochemistry(paraffin):
      1:200 dilution of a previous lot was used on formalin fixed, paraffin embedded tissue sections.
      Biological Information
      ImmunogenFusion protein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityHuman Nestin. The antibody shows no reactivity to vimentin or GFAP.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. May cross-react with murine and rat nestin. Other species have not yet been tested
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
      Gene Symbol
      • FLJ21841
      • Nbla00170
      • OTTHUMP00000032198
      • nestin
      Non-Reactive Species
      • Mouse
      • Rat
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).
      SIZE: 1621 amino acids; 177439 Da
      SUBUNIT: Interacts with FHOD3 (By similarity).
      TISSUE SPECIFICITY: CNS stem cells.
      DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
      SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
      Stem Cell Type
      • Neural Stem Cells
      • Epithelial Cells
      Molecular Weight200-220 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nestin Antibody Certificates of Analysis

      TitleLot Number
      Anti-Nestin - LV1635955LV1635955
      Anti-Nestin - LV1751778LV1751778
      Anti-Nestin - NG1853940NG1853940
      RABBIT ANTI-NESTIN POLYCLONAL ANTIBODY - LV1519217LV1519217

      References

      Reference overviewApplicationSpeciesPub Med ID
      Astroblastoma: beside being a tumor entity, an occasional phenotype of astrocytic gliomas?
      Mellai, M; Piazzi, A; Casalone, C; Grifoni, S; Melcarne, A; Annovazzi, L; Cassoni, P; Denysenko, T; Valentini, MC; Cistaro, A; Schiffer, D
      OncoTargets and therapy  8  451-60  2015

      Show Abstract
      Immunofluorescence25737639 25737639
      Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation.
      Rong, JU; Wen, Z; Rong, WU; Zhichun, F
      Biomedical reports  3  242-246  2015

      Show Abstract
      25798249 25798249
      Properties of neurons derived from induced pluripotent stem cells of Gaucher disease type 2 patient fibroblasts: potential role in neuropathology.
      Sun, Y; Florer, J; Mayhew, CN; Jia, Z; Zhao, Z; Xu, K; Ran, H; Liou, B; Zhang, W; Setchell, KD; Gu, J; Grabowski, GA
      PloS one  10  e0118771  2015

      Show Abstract
      Immunofluorescence25822147 25822147
      A Perturbed MicroRNA Expression Pattern Characterizes Embryonic Neural Stem Cells Derived from a Severe Mouse Model of Spinal Muscular Atrophy (SMA).
      Luchetti, A; Ciafrè, SA; Murdocca, M; Malgieri, A; Masotti, A; Sanchez, M; Farace, MG; Novelli, G; Sangiuolo, F
      International journal of molecular sciences  16  18312-27  2015

      Show Abstract
      26258776 26258776
      Engraftment of Human Glioblastoma Cells in Immunocompetent Rats through Acquired Immunosuppression.
      Huszthy, PC; Sakariassen, PØ; Espedal, H; Brokstad, KA; Bjerkvig, R; Miletic, H
      PloS one  10  e0136089  2015

      Show Abstract
      26291724 26291724
      Synaptic dysregulation in a human iPS cell model of mental disorders.
      Wen, Z; Nguyen, HN; Guo, Z; Lalli, MA; Wang, X; Su, Y; Kim, NS; Yoon, KJ; Shin, J; Zhang, C; Makri, G; Nauen, D; Yu, H; Guzman, E; Chiang, CH; Yoritomo, N; Kaibuchi, K; Zou, J; Christian, KM; Cheng, L; Ross, CA; Margolis, RL; Chen, G; Kosik, KS; Song, H; Ming, GL
      Nature  2014

      Show Abstract
      25132547 25132547
      Contribution of nestin positive esophageal squamous cancer cells on malignant proliferation, apoptosis, and poor prognosis.
      Zhong, B; Wang, T; Lun, X; Zhang, J; Zheng, S; Yang, W; Li, W; Xiang, AP; Chen, Z
      Cancer cell international  14  57  2014

      Show Abstract
      24966803 24966803
      The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress.
      Chang, YC; Chang, WC; Hung, KH; Yang, DM; Cheng, YH; Liao, YW; Woung, LC; Tsai, CY; Hsu, CC; Lin, TC; Liu, JH; Chiou, SH; Peng, CH; Chen, SJ
      Frontiers in aging neuroscience  6  191  2014

      Show Abstract
      25136316 25136316
      Melanoma spheroid formation involves laminin-associated vasculogenic mimicry.
      Larson, AR; Lee, CW; Lezcano, C; Zhan, Q; Huang, J; Fischer, AH; Murphy, GF
      The American journal of pathology  184  71-8  2014

      Show Abstract
      24332013 24332013
      Existence of a potential neurogenic system in the adult human brain.
      Nogueira, AB; Sogayar, MC; Colquhoun, A; Siqueira, SA; Nogueira, AB; Marchiori, PE; Teixeira, MJ
      Journal of translational medicine  12  75  2014

      Show Abstract
      24655332 24655332

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition (MilliporeSigma)

      Data Sheet

      Title
      RABBIT ANTI-NESTIN POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies