IM35 Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5)

IM35
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      IM35-100UG
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      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
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          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.
          Catalogue NumberIM35
          Brand Family Calbiochem®
          Application Data
          Detection of human MMP-1 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) (Cat. No. IM35) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G., et al. 1993. FASEB 7, 1434.
          Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
          Woessner, J.F. 1991. FASEB 5, 2145.
          Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
          Product Information
          DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
          FormLiquid
          FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesParaffin Sections Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1. Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69.
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesFrozen sections (see application references)
          Immunoblotting (1 µg/ml)
          Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
          Application CommentsTo prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Does not cross-react with bovine. Will not cross-react with human MMP-2, MMP-3, MMP-8, MMP-9, or MMP-13. For best results with paraffin sections, pre-treat with a pressure cooker; however, heat, saponin or trypsin can be used in place of a pressure cooker. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (VQGQNVLHGYPKDIYSSFG) corresponding to amino acids 332-350 of human MMP-1
          ImmunogenHuman
          Clone41-1E5
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) Certificates of Analysis

          TitleLot Number
          IM35

          References

          Reference overview
          Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G., et al. 1993. FASEB 7, 1434.
          Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
          Woessner, J.F. 1991. FASEB 5, 2145.
          Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-October-2007 JSW
          ApplicationFrozen sections (see application references)
          Immunoblotting (1 µg/ml)
          Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
          Application Data
          Detection of human MMP-1 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) (Cat. No. IM35) (1 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.
          BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-1 (interstitial collagenase, tissue collagenase, fibroblast collagenase) is secreted as a 57/52 kDa zymogen which is proteolytically processed to the 46/42 kDa active forms. This enzyme displays substrate specificity toward type I, II, III, VII, VIII and X collagens and gelatin. MMP-1 is thought to play an important role in pathophysiological degradation processes associated with conditions such as rheumatoid arthritis, osteoarthritis, and cancer cell invasion.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic peptide (VQGQNVLHGYPKDIYSSFG) corresponding to amino acids 332-350 of human MMP-1
          Clone41-1E5
          IsotypeIgG2a
          Speciesnot bovine, human
          FormLiquid
          FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsTo prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Does not cross-react with bovine. Will not cross-react with human MMP-2, MMP-3, MMP-8, MMP-9, or MMP-13. For best results with paraffin sections, pre-treat with a pressure cooker; however, heat, saponin or trypsin can be used in place of a pressure cooker. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G., et al. 1993. FASEB 7, 1434.
          Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
          Woessner, J.F. 1991. FASEB 5, 2145.
          Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
          Application referencesParaffin Sections Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1. Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69.