434629 Anti-Lewis b Mouse mAb (T218)

434629
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      434629-1ML
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      Stocked 
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          Plastic ampoule 1 ml
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          Description
          OverviewRecognizes the Lewis b carbohydrate chain in colon adenocarcinoma.
          Catalogue Number434629
          Brand Family Calbiochem®
          SynonymsAnti-Leb
          References
          ReferencesCordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
          Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
          Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
          Product Information
          FormLiquid
          FormulationIn PBS, 1% BSA.
          Positive controlColon adenocarcinoma tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesELISA (see comments)
          Immunoblotting (see comments)
          Immunohistochemistry (1:10-1:40)
          Application CommentsFor immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.
          Biological Information
          Immunogenhuman SK-CO-10 colon cancer cells
          ImmunogenHuman
          CloneT218
          HostMouse
          IsotypeIgM
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          434629

          References

          Reference overview
          Cordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
          Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
          Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-August-2007 RFH
          SynonymsAnti-Leb
          ApplicationELISA (see comments)
          Immunoblotting (see comments)
          Immunohistochemistry (1:10-1:40)
          DescriptionPurified mouse monoclonal antibody. Recognizes the Lewis b carbohydrate chain.
          BackgroundBlood group antigens are a group of molecules formed by sequential addition of saccharides to carbohydrate side chains of lipids and proteins and are detected on erythrocytes, certain epithelial cells and in secretions of some individuals. A, B and H antigens are synthesized by erythrocytes and integrated into their cell membranes. Lewis antigens are not synthesized by erythrocytes but can be absorbed from the plasma onto the surface of red blood cells. Subtle differences in the composition and arrangement of the sugars in the chains are sufficient to generate these distinct specificities. Lewis b may be detected in gastrointestinal and colorectal tumors and pancreatic cancer.
          HostMouse
          Immunogen speciesHuman
          Immunogenhuman SK-CO-10 colon cancer cells
          CloneT218
          IsotypeIgM
          Specieshuman
          Positive controlColon adenocarcinoma tissue
          FormLiquid
          FormulationIn PBS, 1% BSA.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsFor immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesCordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
          Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
          Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.