07-432 Anti-K+/Cl- Cotransporter (KCC2) Antibody

07-432
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, Ca ICC, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-432
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-K+/Cl- Cotransporter (KCC2) Antibody
      Alternate Names
      • Electroneutral potassium-chloride cotransporter 2
      • Erythroid K-Cl cotransporter 2
      • Neuronal K-Cl cotransporter
      • electroneural potassium-chloride cotransporter 2
      • solute carrier family 12 (potassium-chloride transporter), member 5
      Background InformationKCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain membrane preparation.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
      Applications
      ApplicationThis Anti-K+/Cl-Cotransporter (KCC2) Antibody is validated for use in IC, WB for the detection of K+/Cl-Cotransporter (KCC2) with more than 15 product citations.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      This antibody has been reported by an independent laboratory to detect K+/Cl- Cotransporter (KCC2) in Madin Darby Canine Kidney (MDCK) cells at 1:200 dilution.
      Biological Information
      ImmunogenN-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140 kDa. An unknown band >200 kDa was also detected.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Canine
      Species Reactivity NoteRat and dog. Wide range of species cross-reactivity expected due to sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryK-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons.
      Gene Symbol
      • SLC12A5
      • KIAA1176
      • hKCC2
      • KCC2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9H2X9 # Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
      SIZE: 1139 amino acids; 126184 Da
      SUBUNIT: Homomultimer and heteromultimer with other K-Cl cotransporters (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Brain specific. Detected in neuronal cells.
      SIMILARITY:SwissProt: Q9H2X9 ## Belongs to the SLC12A transporter family.
      MISCELLANEOUS: Inhibited by furosemide and bumetanide.
      Molecular Weight140 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot in rat brain membrane preperations.

      Western Blot Analysis: 1.0 µg/mL of this lot detected K+/Cl- Cotransporter (KCC2) in rat brain membrane preparations.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-K+/Cl- Cotransporter (KCC2) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-K+/Cl- Cotransporter (KCC2) Antibody Certificates of Analysis

      TitleLot Number
      Anti-K+/Cl- Cotransporter (KCC2) - 21201282120128
      Anti-K+/Cl- Cotransporter (KCC2) - 23733892373389
      Anti-K+/Cl- Cotransporter (KCC2) - 24525232452523
      Anti-K+/Cl- Cotransporter (KCC2) - 19592671959267
      Anti-K+/Cl- Cotransporter (KCC2) - 20196012019601
      Anti-K+/Cl- Cotransporter (KCC2) - 20685662068566
      Anti-K+/Cl- Cotransporter (KCC2) - 21931612193161
      Anti-K+/Cl- Cotransporter (KCC2) - 22804192280419
      Anti-K+/Cl- Cotransporter (KCC2) - 24651972465197
      Anti-K+/Cl- Cotransporter (KCC2) - 2730897A2730897A

      References

      Reference overviewApplicationSpeciesPub Med ID
      Age- and sex-dependent susceptibility to phenobarbital-resistant neonatal seizures: role of chloride co-transporters.
      Kang, SK; Markowitz, GJ; Kim, ST; Johnston, MV; Kadam, SD
      Frontiers in cellular neuroscience  9  173  2015

      Show Abstract
      26029047 26029047
      Early depolarizing GABA controls critical-period plasticity in the rat visual cortex.
      Deidda, G; Allegra, M; Cerri, C; Naskar, S; Bony, G; Zunino, G; Bozzi, Y; Caleo, M; Cancedda, L
      Nature neuroscience  18  87-96  2015

      Show Abstract
      25485756 25485756
      Nitric oxide signaling modulates synaptic inhibition in the superior paraolivary nucleus (SPN) via cGMP-dependent suppression of KCC2.
      Yassin, L; Radtke-Schuller, S; Asraf, H; Grothe, B; Hershfinkel, M; Forsythe, ID; Kopp-Scheinpflug, C
      Frontiers in neural circuits  8  65  2014

      Show Abstract
      Immunofluorescence24987336 24987336
      Overexpression of BDNF increases excitability of the lumbar spinal network and leads to robust early locomotor recovery in completely spinalized rats.
      Ziemlińska, E; Kügler, S; Schachner, M; Wewiór, I; Czarkowska-Bauch, J; Skup, M
      PloS one  9  e88833  2014

      Show Abstract
      24551172 24551172
      Gephyrin clusters are absent from small diameter primary afferent terminals despite the presence of GABA(A) receptors.
      Lorenzo, LE; Godin, AG; Wang, F; St-Louis, M; Carbonetto, S; Wiseman, PW; Ribeiro-da-Silva, A; De Koninck, Y
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  8300-17  2014

      Show Abstract
      24920633 24920633
      Synaptic elements for GABAergic feed-forward signaling between HII horizontal cells and blue cone bipolar cells are enriched beneath primate S-cones.
      Puller, C; Haverkamp, S; Neitz, M; Neitz, J
      PloS one  9  e88963  2014

      Show Abstract
      24586460 24586460
      Down-regulation of KCC2 expression and phosphorylation in motoneurons, and increases the number of in primary afferent projections to motoneurons in mice with post-stroke spasticity.
      Toda, T; Ishida, K; Kiyama, H; Yamashita, T; Lee, S
      PloS one  9  e114328  2014

      Show Abstract
      25546454 25546454
      Immunolocalization of cation-chloride cotransporters in the developing and mature spinal cord of opossums, Monodelphis domestica.
      Phan, HL; Pflieger, JF
      Frontiers in neuroanatomy  7  12  2013

      Show Abstract
      23720613 23720613
      An unexpected role of neuroligin-2 in regulating KCC2 and GABA functional switch.
      Sun, C; Zhang, L; Chen, G
      Molecular brain  6  23  2013

      Show Abstract
      23663753 23663753
      Decreased Immunoreactivities of the Chloride Transporters, KCC2 and NKCC1, in the Lateral Superior Olive Neurons of Kanamycin-treated Rats.
      Suh, MW; Ahn, SC
      Clinical and experimental otorhinolaryngology  5  117-21  2012

      Show Abstract
      22977707 22977707

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      Categories

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