407143 Anti-IP₃ Receptor Rabbit pAb

407143
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, Ca, H, M, Porcine, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      407143-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes all of the ~260 kDa IP3 receptor subtypes.
          Catalogue Number407143
          Brand Family Calbiochem®
          References
          ReferencesBourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
          Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
          Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunofluorescence
          Immunohistochemistry
          Immunoprecipitation
          Radioimmunoassay
          Enzyme-Linked Immunosorbent Assay
          Flow Cytometry
          Immunoblotting (Western Blotting)
          Application NotesELISA (1:2000)
          Flow Cytometry (1:1000)
          Immunoblotting (1:250)
          Immunofluorescence (1:200)
          Immunohistochemistry (1:200)
          Immunoprecipitation (1:200)
          Radioimmunoassay (1:10000)
          Application CommentsVariables associated with assay conditions will dictate the proper working dilution.

          Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies

          NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.

          • TBST
          • 20 mM Tris-HCl, pH 7.6
          • 150 mM NaCl
          • 0.05% Tween®-20 detergent

          1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
          2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
          3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
          4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
          5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
          6. Shake for 1 h.
          7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
          8. Mix development solution (1:1) (Amersham ECL kit).
          9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Bovine
          • Canine
          • Human
          • Mouse
          • Porcine
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          407143

          References

          Reference overview
          Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
          Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
          Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Calcium Metabolism and Related Products Brochure
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-August-2007 RFH
          ApplicationELISA (1:2000)
          Flow Cytometry (1:1000)
          Immunoblotting (1:250)
          Immunofluorescence (1:200)
          Immunohistochemistry (1:200)
          Immunoprecipitation (1:200)
          Radioimmunoassay (1:10000)
          DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP3 receptor protein.
          BackgroundInositol 1,4,5-triphosphate (IP3) receptors have been shown to play an important role in the regulation of Ca2+ release from internal storage sites during cell activation.
          HostRabbit
          Immunogena synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
          IsotypeIgG
          Speciesbovine, canine, human, mouse, porcine, rat
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsVariables associated with assay conditions will dictate the proper working dilution.

          Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies

          NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.

          • TBST
          • 20 mM Tris-HCl, pH 7.6
          • 150 mM NaCl
          • 0.05% Tween®-20 detergent

          1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
          2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
          3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
          4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
          5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
          6. Shake for 1 h.
          7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
          8. Mix development solution (1:1) (Amersham ECL kit).
          9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
          Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
          Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.