OP133 Anti-IKKα Mouse mAb (14A231)

OP133
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, Mk, M M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP133-100UG
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~85 kDa IKKα protein in Daudi cells.
          Catalogue NumberOP133
          Brand Family Calbiochem®
          Application Data
          Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesZandi, E., et al. 1998 Science 281, 1360.
          Didonato, J.A., et al. 1997 Nature 388, 548.
          Lee, F.S., et al. 1997 Cell 88, 213.
          Mercurio, F., et al. 1997 Science 278, 860.
          Regnier, C.H., et al. M. 1997 Cell 90, 373.
          Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
          Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.
          Product Information
          FormLiquid
          FormulationIn PBS, 0.2% gelatin, pH 7.4.
          Positive controlDaudi cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag® sequence
          ImmunogenHuman
          Clone14A231
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Monkey
          • Mouse
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-IKKα Mouse mAb (14A231) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-IKKα Mouse mAb (14A231) Certificates of Analysis

          TitleLot Number
          OP133

          References

          Reference overview
          Zandi, E., et al. 1998 Science 281, 1360.
          Didonato, J.A., et al. 1997 Nature 388, 548.
          Lee, F.S., et al. 1997 Cell 88, 213.
          Mercurio, F., et al. 1997 Science 278, 860.
          Regnier, C.H., et al. M. 1997 Cell 90, 373.
          Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
          Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2007 RFH
          ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
          Application Data
          Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.
          BackgroundNF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β and IKK3/γ. In vitro, IKK1/α and IKK2/β can form homo- and heterodimers that can phosphorylate IκBs at the regulatory serine residues directly. IKK1/α and IKK2/β are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/α gene in mice results in skin and limb abnormalities and death of newborns.
          HostMouse
          Immunogen speciesHuman
          Immunogena recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag® sequence
          Clone14A231
          IsotypeIgG₁
          Specieshuman, monkey, mouse
          Positive controlDaudi cells
          FormLiquid
          FormulationIn PBS, 0.2% gelatin, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesZandi, E., et al. 1998 Science 281, 1360.
          Didonato, J.A., et al. 1997 Nature 388, 548.
          Lee, F.S., et al. 1997 Cell 88, 213.
          Mercurio, F., et al. 1997 Science 278, 860.
          Regnier, C.H., et al. M. 1997 Cell 90, 373.
          Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
          Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.