386041 | Anti-Hsp90 Rat mAb (16F1)

386041
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species R Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      386041-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes the ~90 kDa Hsp90 protein.
          Catalogue Number386041
          Brand Family Calbiochem®
          SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
          Application Data
          Detection of Hsp90 by immunoblotting. Samples: Molecular weight marker (lane 1), Hsp90 native protein (lane 2), Hsp90 recombinant protein (lane 3), Hsp90 alpha recombinant protein (lane 4), heat shocked HeLa (lane 5), L-929 (lane 6), Rat-2 cells (lane 7), and RK-13 cells (lane 8). Primary antibody: Anti-Hsp90 Rat mAb (16F1) (Cat. No. 386041) (1:1000). Detection: chemiluminescence.
          References
          ReferencesSchuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
          Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
          Lanks, K.W., et al. 1982. J. Biol. Chem. 257, 8604.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol.
          Positive controlHeat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunohistochemistry
          Immunoprecipitation
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (2 µg/ml, colormetric)
          Immunocytochemistry (see comments)
          Immunohistochemistry (see comments)
          Immunoprecipitation (see comments)
          Electron Microscopy (see comments)
          Application CommentsThis antibody also works in plants, specifically cotton, by immunoblot analysis. Has also been reported to work for immunoprecipitation, ELISA, immunohistochemistry, immunocytochemistry, and electron microscopy. Note: Rat immunoglobulins do not react well with protein A. For immunoprecipitation, protein G is recommended. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenHsp90 isolated from therapeutic human orchiectomy specimens
          ImmunogenHuman
          Clone16F1
          HostRat
          IsotypeIgG2a
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          386041

          References

          Reference overview
          Schuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
          Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
          Lanks, K.W., et al. 1982. J. Biol. Chem. 257, 8604.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision11-November-2011 JSW
          SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
          ApplicationELISA (see comments)
          Immunoblotting (2 µg/ml, colormetric)
          Immunocytochemistry (see comments)
          Immunohistochemistry (see comments)
          Immunoprecipitation (see comments)
          Electron Microscopy (see comments)
          Application Data
          Detection of Hsp90 by immunoblotting. Samples: Molecular weight marker (lane 1), Hsp90 native protein (lane 2), Hsp90 recombinant protein (lane 3), Hsp90 alpha recombinant protein (lane 4), heat shocked HeLa (lane 5), L-929 (lane 6), Rat-2 cells (lane 7), and RK-13 cells (lane 8). Primary antibody: Anti-Hsp90 Rat mAb (16F1) (Cat. No. 386041) (1:1000). Detection: chemiluminescence.
          DescriptionProtein G purified rat monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
          BackgroundHsp90 (heat shock protein 90) is a primarily cytoplasmic protein which exists in dimeric form. Hsp90 binds several other cellular proteins such as retrovirus kinases (pp60v-src), steroid receptors, heme-regulated protein kinase, actin, and tubulin. Although Hsp90 expression is induced by stress, and thus it may be an indicator of cell stress, its constitutive level may be as high as 1-2% of the total extractable cellular protein.
          HostRat
          Immunogen speciesHuman
          ImmunogenHsp90 isolated from therapeutic human orchiectomy specimens
          Clone16F1
          IsotypeIgG2a
          Speciesa broad range of species
          Positive controlHeat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells
          FormLiquid
          FormulationIn PBS, 50% glycerol.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThis antibody also works in plants, specifically cotton, by immunoblot analysis. Has also been reported to work for immunoprecipitation, ELISA, immunohistochemistry, immunocytochemistry, and electron microscopy. Note: Rat immunoglobulins do not react well with protein A. For immunoprecipitation, protein G is recommended. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesSchuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
          Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
          Lanks, K.W., et al. 1982. J. Biol. Chem. 257, 8604.