400064 | Anti-Glucose Transporter-4 Rabbit pAb

400064
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      400064-50UG
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes the ~40-43 kDa GLUT-4 protein.
          Catalogue Number400064
          Brand Family Calbiochem®
          SynonymsAnti-Glut-4
          References
          ReferencesMueckler, M. 1994. Eur. J. Biochem. 219, 713.
          James, D.E., et al. 1989. Nature 338, 83.
          James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
          Product Information
          FormLiquid
          FormulationIn PBS, 0.1% BSA, pH 7.5.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesELISA (0.5-1 µg/ml)
          Frozen Sections (2-20 µg/ml)
          Immunoblotting (1-10 µg/ml; see comments)
          Immunoprecipitation (1-10 µg/100 µg tissue membrane)
          Application CommentsDoes not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
          ImmunogenMouse
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          400064

          References

          Reference overview
          Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
          James, D.E., et al. 1989. Nature 338, 83.
          James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-March-2009 RFH
          SynonymsAnti-Glut-4
          ApplicationELISA (0.5-1 µg/ml)
          Frozen Sections (2-20 µg/ml)
          Immunoblotting (1-10 µg/ml; see comments)
          Immunoprecipitation (1-10 µg/100 µg tissue membrane)
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.
          BackgroundGlucose transporter-4 (Glut-4) is a transmembrane protein that facilitates transport of glucose in the cell in an energy-independent fashion. Insulin activates glucose transport in fat cells through a translocation of the insulin-sensitive Glut-4 and by activation of glucose carriers in the plasma membrane. Glut-4 is unique to tissues that exhibit insulin-stimulated glucose uptake (heart and skeletal muscle and adipose tissue).
          HostRabbit
          Immunogen speciesMouse
          Immunogena synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
          IsotypeIgG
          Specieshuman, mouse, rat
          FormLiquid
          FormulationIn PBS, 0.1% BSA, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsDoes not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesMueckler, M. 1994. Eur. J. Biochem. 219, 713.
          James, D.E., et al. 1989. Nature 338, 83.
          James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.