MABE606 Anti-GAPDH, clone EPR6256, Rabbit Monoclonal Antibody

MABE606
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC, ICC Rb Unpurified Monoclonal Antibody
      Description
      Catalogue NumberMABE606
      DescriptionAnti-GAPDH, clone EPR6256, Rabbit Monoclonal Antibody
      Alternate Names
      • Glyceraldehyde-3-phosphate dehydrogenase
      • GAPDH
      • Peptidyl-cysteine S-nitrosylase GAPDH
      Background InformationGlyceraldehyde-3-phosphate dehydrogenase or GAPDH or Peptidyl-cysteine S-nitrosylase GAPDH, and encoded by the gene name GAPDH or GAPD, is an ubiquitous glycolytic enzyme present in moderately high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.
      Applications
      ApplicationThis Anti-GAPDH antibody is validated for use in WB, FC, IC for the detection of GAPDH.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesFlow Cytometry Analysis: A 1:100-1,000 dilution from a representative lot detected GAPDH in HeLa cells.

      Immunocytochemistry Analysis: A 1:250-500 dilution from a representative lot detected GAPDH in MCF7 cells.

      Produced in collaboration with Epitomics™
      Biological Information
      ImmunogenSynthetic peptide corresponding to human GAPDH.
      CloneEPR6256
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • GAPDH
      • GAPD
      • CDABP0047
      • OK/SW-cl.12
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~36 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293, HeLa, HepG2, HUVEC, MCF7 (lance 5), and SH-SY5Y cell lysates.

      Western Blotting Analysis: A 1:10,000-50,000 dilution of this antibody detected GAPDH in HEK293, HeLa, HepG2, HUVEC, MCF7 (lance 5), and SH-SY5Y cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications