09-071 Anti-G9a (BAT8) Antibody

09-071
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ca WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number09-071
      DescriptionAnti-G9a (BAT8) Antibody
      Alternate Names
      • G9A histone methyltransferase
      • H3-K9-HMTase 3
      • HLA-B associated transcript 8
      • HLA-B-associated transcript 8
      • Histone H3-K9 methyltransferase 3
      • Lysine N-methyltransferase 1C
      • Protein G9a
      • ankyrin repeat-containing protein
      • chromosome 6 open reading frame 30
      • euchromatic histone-lysine N-methyltransferase 2
      Background InformationG9a (BAT8 or Histone-lysine N-methyltransferase) is thought to be involved in intracellular protein-protein interaction and is a lysine-preferring histone methyltransferase. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. The presence of the ankyrin repeats also suggests that G9a is involved in intracellular protein-protein interaction. Like Suv39h1, G9a transfers methyl groups to the lysine residues of histone H3, but with a 10- to 20-fold higher activity. G9a is localized to the nucleus but not in the repressive chromatin domains of centromeric loci, in which Suv39h1 family proteins were localized. This protein is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a also methylates histone H1.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
      Applications
      ApplicationAnti-G9a (BAT8) Antibody is a rabbit polyclonal antibody for detection of G9a (BAT8) also known as G9A histone methyltransferase ,H3-K9-HMTase 3 ,HLA-B associated transcript 8 & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis (SNAP ID Technique): 1 µg/mL from a previous lot detected G9a (BAT8) on 10 µg of HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to G9a (BAT8).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes G9a (BAT8).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      Species Reactivity NoteProven to react with human and mouse. Predicted to react with rat and canine based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described.
      Gene Symbol
      • BAT8
      • C6orf30
      • DKFZp686H08213
      • EC 2.1.1.43
      • Em:AF134726.3
      • FLJ35547
      • G9A
      • KMT1C
      • NG36
      • NG36/G9a
      • OTTHUMP00000029262
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1 By similarity.
      CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
      SUBUNIT STRUCTURE: Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1.
      SUBCELLULAR LOCATION: Nucleus. Note: Associates with euchromatic regions. Does not associate with heterochromatin.
      TISSUE SPECIFICITY: Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
      DOMAIN: The SET domain mediates interaction with WIZ.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
      Contains 7 ANK repeats.
      Contains 1 post-SET domain.
      Contains 1 pre-SET domain.
      Contains 1 SET domain.
      CAUTION: reported that while NG36 and G9a were originally thought to derive from two separate genes, all G9A transcripts also contain the in frame coding sequence of NG36.
      It is uncertain whether Met-1 or Met-21 is the initiator methionine.
      SEQUENCE CAUTION: The sequence AAD21811.1 differs from that shown. Reason: Erroneous gene model prediction.
      The sequence AAD21812.1 differs from that shown. Reason: Erroneous gene model prediction.
      The sequence BAB63294.1 differs from that shown. Reason: Erroneous gene model prediction.
      The sequence BAB63295.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight~160 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.
      Western Blot Analysis: : 0.25-2 µg/mL of this antibody detected G9a (BAT8) in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-G9a (BAT8) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-G9a (BAT8) Antibody Certificates of Analysis

      TitleLot Number
      Anti-G9a (BAT8)2470749
      Anti-G9a (BAT8) - 24204132420413
      Anti-G9a (BAT8) - 24532422453242
      Anti-G9a (BAT8) - 20382952038295
      Anti-G9a (BAT8) - 22071932207193
      Anti-G9a (BAT8) - 25124562512456
      Anti-G9a (BAT8) - NG1627073NG1627073
      Anti-G9a (BAT8) - NG1820230NG1820230
      Anti-G9a (BAT8) - NG1894429NG1894429
      Anti-G9a (BAT8) -25759122575912

      References

      Reference overviewApplicationPub Med ID
      Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice.
      Subbanna, S; Nagre, NN; Shivakumar, M; Umapathy, NS; Psychoyos, D; Basavarajappa, BS
      Neuroscience  258  422-32  2014

      Show Abstract
      Immunoprecipitation24300108 24300108
      G9a influences neuronal subtype specification in striatum.
      Maze, I; Chaudhury, D; Dietz, DM; Von Schimmelmann, M; Kennedy, PJ; Lobo, MK; Sillivan, SE; Miller, ML; Bagot, RC; Sun, H; Turecki, G; Neve, RL; Hurd, YL; Shen, L; Han, MH; Schaefer, A; Nestler, EJ
      Nature neuroscience  17  533-9  2014

      Show Abstract
      Immunohistochemistry24584053 24584053
      G9a-mediated histone methylation regulates ethanol-induced neurodegeneration in the neonatal mouse brain.
      Subbanna, S; Shivakumar, M; Umapathy, NS; Saito, M; Mohan, PS; Kumar, A; Nixon, RA; Verin, AD; Psychoyos, D; Basavarajappa, BS
      Neurobiology of disease  54  475-85  2013

      Show Abstract
      Western Blotting23396011 23396011
      Histone H3K9 methyltransferase G9a represses PPARγ expression and adipogenesis.
      Wang, L; Xu, S; Lee, JE; Baldridge, A; Grullon, S; Peng, W; Ge, K
      The EMBO journal  32  45-59  2013

      Show Abstract
      Western Blotting23178591 23178591

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition (MilliporeSigma)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies