MAB3073 Anti-G Protein Goα Antibody, clone 2A

MAB3073
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Gp, H, M, R WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3073
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-G Protein Goα Antibody, clone 2A
      References
      Product Information
      FormatPurified
      PresentationPurified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl, pH 7.6, containing 0.1% sodium azide as a preservative.
      Applications
      ApplicationAnti-G Protein Goα Antibody, clone 2A is an antibody against G Protein Goα for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot 1:3000.

      Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenBovine brain Go-alpha purified by the method of Sternweis and Robishaw (J. Biol. Chem. [1984] 259, 13806).
      Clone2A
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityGo-alpha. Strong reactivity with bovine (100%), human (70%), rat (61%) and mouse (54%). Slight reactivity with guinea pig (27%). Specificity confirmed by Western blot versus bovine G-alpha-t, rat Go-alpha, rat Gi-alpha-1, Gi-alpha-2, rat Gi-alpha-3, bovine Gs-alpha, and murine Go-alpha-A and Go-alpha-B. The carboxy-terminal splice variants of Go-alpha (Go-alpha-A and Go-alpha-B) expressed in bacterial lysates were compared for reactivity and found to be equal . As Go-alpha-A and Go-alpha-B have >95% homology to rat Go-alpha-1 and human Go-alpha-2, MAB3073 will detect these G-proteins also.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Guinea Pig
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GNAO1
      • DKFZp686O0962
      • G-ALPHA-o
      • GNAO
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29777 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear.| P09471 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear.
      SIZE: 354 amino acids; 40087 Da
      SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
      DOMAIN: SwissProt: P29777
      SIMILARITY: SwissProt: P29777 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily. | P09471 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for upt to 6 months after date of receipt.

      During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-G Protein Goα Antibody, clone 2A SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-G Protein Goα Antibody, clone 2A Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-Goa MONOCLONAL ANTIBODY2942490
      MOUSE ANTI-Goa MONOCLONAL ANTIBODY - 20647092064709
      MOUSE ANTI-Goa MONOCLONAL ANTIBODY - 21812702181270
      MOUSE ANTI-Goa MONOCLONAL ANTIBODY - 23263852326385
      MOUSE ANTI-Goα - 25006342500634
      MOUSE ANTI-Goα -25576602557660
      MOUSE ANTI-Goα -27244572724457
      MOUSE ANTI-Goα -27503172750317
      MOUSE ANTI-Goα -28404662840466
      MOUSE ANTI-Goα MONOCLONAL ANTIBODY3043674

      References

      Reference overviewApplicationSpeciesPub Med ID
      Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool.
      van Wyk, M; Pielecka-Fortuna, J; Löwel, S; Kleinlogel, S
      PLoS biology  13  e1002143  2015

      Show Abstract
      Immunoblotting (Western)25950461 25950461
      Oligomeric state of purified transient receptor potential melastatin-1 (TRPM1), a protein essential for dim light vision.
      Agosto, MA; Zhang, Z; He, F; Anastassov, IA; Wright, SJ; McGehee, J; Wensel, TG
      The Journal of biological chemistry  289  27019-33  2014

      Show Abstract
      Immunoblotting (Western)25112866 25112866
      Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter.
      Cronin, T; Vandenberghe, LH; Hantz, P; Juttner, J; Reimann, A; Kacsó, AE; Huckfeldt, RM; Busskamp, V; Kohler, H; Lagali, PS; Roska, B; Bennett, J
      EMBO molecular medicine  6  1175-90  2014

      Show Abstract
      Immunoblotting (Western)25092770 25092770
      p53 selectively regulates developmental apoptosis of rod photoreceptors.
      Vuong, L; Brobst, DE; Ivanovic, I; Sherry, DM; Al-Ubaidi, MR
      PloS one  8  e67381  2013

      Show Abstract
      23840687 23840687
      Expression of voltage-gated calcium channel α(2)δ(4) subunits in the mouse and rat retina.
      De Sevilla Müller, LP; Liu, J; Solomon, A; Rodriguez, A; Brecha, NC
      The Journal of comparative neurology  521  2486-501  2013

      Show Abstract
      23296739 23296739
      Metabotropic glutamate receptor 6 signaling enhances TRPM1 calcium channel function and increases melanin content in human melanocytes.
      Devi, S; Markandeya, Y; Maddodi, N; Dhingra, A; Vardi, N; Balijepalli, RC; Setaluri, V
      Pigment cell & melanoma research  26  348-56  2013

      Show Abstract
      23452348 23452348
      Quantitative confocal imaging of the retinal microvasculature in the human retina.
      Tan, PE; Yu, PK; Balaratnasingam, C; Cringle, SJ; Morgan, WH; McAllister, IL; Yu, DY
      Investigative ophthalmology & visual science  53  5728-36  2012

      Show Abstract
      22836777 22836777
      Gβ3 is required for normal light on responses and synaptic maintenance.
      Anuradha Dhingra,Hariharasubramanian Ramakrishnan,Adam Neinstein,Marie E Fina,Ying Xu,Jian Li,Daniel C Chung,Arkady Lyubarsky,Noga Vardi
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  2012

      Show Abstract
      22895717 22895717
      mGluR6 deletion renders the TRPM1 channel in retina inactive.
      Ying Xu,Anuradha Dhingra,Marie E Fina,Chieko Koike,Takahisa Furukawa,Noga Vardi
      Journal of neurophysiology  107  2012

      Show Abstract
      22131384 22131384
      Melatonin receptors are anatomically organized to modulate transmission specifically to cone pathways in the retina of Xenopus laevis.
      Allan F Wiechmann,David M Sherry
      The Journal of comparative neurology  520  2012

      Show Abstract
      22020534 22020534

      Data Sheet

      Title
      Anti-G Protein Goalpha, clone 2A - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies