MAB3075 Anti-G Protein Giα-1 Antibody, clone R4

MAB3075
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Gp, H, M, R WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB3075
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-G Protein Giα-1 Antibody, clone R4
      References
      Product Information
      FormatAscites
      PresentationLiquid ascites, containing sodium azide as a preservative.
      Applications
      ApplicationAnti-G Protein Giα-1 Antibody, clone R4 detects level of G Protein Giα-1 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot 1:500 - 1:2000.

      Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRat brain G-proteins purified by the method of Sternweis and Robishaw (Sternweis & Robishaw, 1984).
      CloneR4
      HostMouse
      SpecificityGialpha-1. Strong reactivity with rat (100%), mouse (78%), bovine (74%) and guinea pig (48%). Slight reactivity with human (31%). Specificity confirmed by Western blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.
      IsotypeIgG2b
      Species Reactivity
      • Bovine
      • Guinea Pig
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGuanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]
      Gene Symbol
      • GNAI1
      • Gi
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63096 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.
      SIZE: 354 amino acids; 40361 Da
      SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity).
      SIMILARITY: SwissProt: P63096 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-G Protein Giα-1 Antibody, clone R4 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-Giα−1 -28322262832226
      MOUSE ANTI-Giα−1 MONOCLONAL ANTIBODY3048296
      MOUSE ANTI-Gi1 -27544022754402

      References

      Reference overviewPub Med ID
      Minocycline provides protection against beta-amyloid(25-35)-induced alterations of the somatostatin signaling pathway in the rat temporal cortex.
      E Burgos-Ramos,L Puebla-Jiménez,E Arilla-Ferreiro
      Neuroscience  154  2008

      Show Abstract
      18555616 18555616
      Gly-Pro-Glu protects beta-amyloid-induced somatostatin depletion in the rat cortex.
      David Aguado-Llera, Mercedes Martín-Martínez, María Teresa García-López, Eduardo Arilla-Ferreiro, Vicente Barrios
      Neuroreport  15  1979-82  2004

      Show Abstract
      15305149 15305149
      Acutely administered melatonin decreases somatostatin-binding sites and the inhibitory effect of somatostatin on adenylyl cyclase activity in the rat hippocampus.
      Rosa María Izquierdo-Claros, María del Carmen Boyano-Adánez Md, Eduardo Arilla-Ferreiro
      Journal of pineal research  36  87-94  2004

      Show Abstract
      14962059 14962059
      Activity of the hippocampal somatostatinergic system following daily administration of melatonin.
      Rosa María Izquierdo-Claros, María Del Carmen Boyano-Adánez, Eduardo Arilla-Ferreiro
      Brain research. Molecular brain research  126  107-13  2004

      Show Abstract
      15249133 15249133
      Effects of subchronic and chronic melatonin treatment on somatostatin binding and its effects on adenylyl cyclase activity in the rat frontoparietal cortex.
      Rosa María Izquierdo-Claros, María del Carmen Boyano-Adánez, Eduardo Arilla-Ferreiro
      Journal of pineal research  33  189-97  2002

      Show Abstract
      12390500 12390500
      Acute modulation of somatostatin receptor function by melatonin in the rat frontoparietal cortex.
      R M Izquierdo-Claros, M C Boyano-Adánez, G Torrecillas, M Rodríguez-Puyol, E Arilla-Ferreiro
      Journal of pineal research  31  46-56  2001

      Show Abstract
      11485004 11485004
      Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain.
      Sternweis, P C and Robishaw, J D
      J. Biol. Chem., 259: 13806-13 (1984)  1984

      Show Abstract
      6438083 6438083

      Data Sheet

      Title
      Anti-G Protein Gialpha-1, clone R4 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies