07-1500 Anti-G Protein Giα 1/2 Antibody

07-1500
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information
      Replacement Information07-1500 is a recommended replacement for AB4067

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, B WB, IH(P) Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-1500
      ReplacesAB4067
      DescriptionAnti-G Protein Giα 1/2 Antibody
      Background InformationGTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).
      References
      Product Information
      FormatPurified
      Control
      • L6 Cell Lysate
      PresentationPurified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide.
      Applications
      ApplicationAnti-G Protein Giα 1/2 Antibody is an antibody against G Protein Giα 1/2 for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry(paraffin):
      Representative testing from a previous lot.
      Optimal Staining of GI ALPHA 1/2 With Citrate pH 6.0 Epitope Retrieval: Rat Cerebellum GI ALPHA 1/2 representative staining pattern/morphology in rat cerebellum. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPeptide corresponding to amino acids 345-354 of Gi1 and 346-355 of Gi2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes G protein subunit αi1.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Bovine
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGuanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM] ...
      Gene Symbol
      • GNAI1
      • Gi
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63096 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.
      SIZE: 354 amino acids; 40361 Da
      SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity).
      SIMILARITY: SwissProt: P63096 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
      Molecular Weight~40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on L6 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected G protein Gialpha 1/2 on 10 μg of L6 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20°C in undiluted aliquots for up to 12 months from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Related Products

      Catalog Number Description  
      05-555 Anti-Giα1 Antibody, clone 7H7 Show Pricing & Availability
      06-236 Anti-Giα1/2 Antibody Show Pricing & Availability
      MAB3075 Anti-G Protein Giα-1 Antibody, clone R4 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies