MABT376 Anti-Ero1 Antibody, clone 2G4/12

MABT376
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IP, IF M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT376
      DescriptionAnti-Ero1 Antibody, clone 2G4/12
      Alternate Names
      • ERO1-like protein alpha
      • ERO1-L
      • ERO1-L-alpha
      • Endoplasmic oxidoreductin-1-like protein
      • Oxidoreductin-1-L-alpha
      Background InformationERO1-like protein alpha (ERO1) is also called endoplasmic oxidoreductin-1-like protein and Oxidoreductin-1-L-alpha. ERO1 is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1 is stimulated by hypoxia, indicating that it is regulated by the HIF-pathway. ERO1 may be responsible for much of the reactive oxygen species (ROS) in the cell, and therefore is a source of oxidative stress. EROS1 is required for the folding of immunoglobulin proteins, responsible for the release of the unfolded cholera toxin, and plays an important role in ER stress-induced, CHOP-dependent apoptosis. ERO1 is widely expressed at low levels, with high levels in upper digestive tract.
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Ero1 Antibody, clone 2G4/12 is a highly specific mouse monoclonal antibody, that targets ERO1-like protein alpha & has been tested in western blotting, IP & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected Ero1 from 10 µg of the following cell lysates--A431, HEK293, HeLa, HepG2, human placenta tissue, HUVEC, jurkat, L6, NIH/3T3, PC12, PC3, and RAW264.7.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Ero1 in Ero1 transfected HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).

      Immunofluorescence Analysis: A representative lot detected Ero1 in HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to Human Ero1.
      Clone2G4/12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ERO1L
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Ero1 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications